Multifunctional biodegradable polymer/clay nanocomposites with antibacterial properties in drug delivery systems
Multifunctional biodegradable polymer/clay nanocomposites with antibacterial properties in drug delivery systems
复制标题
DOI：
10.37190/abb-01523-2019-03
复制
发表时间：
2020-01-01
影响因子：
1
通讯作者：
Dudek, Piotr
中科院分区：
文献类型：
--
作者：
Rapacz-Kmita, Alicja;Szaraniec, Barbara;Dudek, Piotr
目的：本研究的目的是调查的可能性，插层的庆大霉素和新霉素在蒙脱土（MMT）纳米填料，以及研究在体外抗菌性能的纳米复合膜含有少量的由此获得的纳米填料。研究方法：将药物在水溶液中插层后，采用流延法制备了药物插层蒙脱土填充的聚乳酸纳米复合膜。通过X射线衍射（XRD）和Zeta电位测量证实了插层效率。在磷酸盐缓冲盐水中孵育6周期间，研究了材料的表面润湿性、粗糙度和机械性能，并在37 ℃下在蒸馏水中孵育6周之前和之后测试了它们对大肠杆菌的杀菌活性。通过电导率和pH测量监测孵育期间抗生素的存在。结果如下：结果表明，纳米复合材料聚乳酸薄膜与蒙脱土填充插入庆大霉素和新霉素倾向于降解速度比他们的同行与非插层填料，这影响了他们的机械性能。然而，药物嵌入提供了抗菌活性，这是通过两种抗生素抑制革兰氏阴性菌生长的区域的存在而证实的。还证实了抗生素与粘土和聚合物基质的相互作用不会对这种杀菌作用产生不利影响。结论：蒙脱石可成功地与庆大霉素和新霉素插层，然后用作具有非常好的抗菌性能的聚乳酸膜的活性填料，因此它们在生物医学应用中的用途可显著扩展。
Purpose: The aim of this study was to investigate the possibility of intercalation of gentamicin and neomycin in montmorillonite (MMT) nanofillers, as well as to study the in vitro antimicrobial properties of nanocomposite films containing a small amount of thus obtained nanofillers. Methods: The polylactide matrix (PLA) nanocomposite films with drug-intercalated montmorillonite fillers were obtained by casting after intercalation of drugs in aqueous solutions. The efficiency of intercalation has been confirmed by X-ray diffraction (XRD) and Zeta potential measurements. The materials were studied for surface wettability, roughness and mechanical properties during 6 weeks of incubation in phosphate buffer saline, and their bactericidal activity was tested against Escherichia coli bacteria before and after 6 weeks of incubation in distilled water at 37 degrees C. The presence of antibiotics during the incubation was monitored by conductivity and pH measurements. Results: The results indicate that nanocomposite polylactide films with montmorillonite filler intercalated with gentamicin and neomycin tend to degrade faster that their counterparts with non-intercalated fillers, which affects their mechanical properties. However, drug intercalation provided an antibacterial activity, which was confirmed by the presence of zones inhibiting the growth of Gram-negative bacteria for both antibiotics. It was also confirmed that the interaction of antibiotics with clay and polymer matrix did not adversely affect this bactericidal effect. Conclusions: Montmorillonite can be successfully intercalated with both gentamicin and neomycin, and then used as active filler for polylactide films having very good antibacterial properties, therefore their use in biomedical applications can be significantly expanded.