A novel class of herpesvirus with bivalve hosts
A novel class of herpesvirus with bivalve hosts
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DOI：
10.1099/vir.0.80382-0
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发表时间：
2005-01-01
影响因子：
3.8
通讯作者：
Renault, T
中科院分区：
文献类型：
--
作者：
Davison, AJ;Trus, BL;Renault, T
牡蛎疱疹病毒1型（Ostreid herpesvirus 1，OsHV-1）是疱疹病毒科中唯一一种以无脊椎动物为宿主的病毒，与太平洋牡蛎（Crassostrea gigas）和其他双壳类动物的偶发性死亡有关。纯化的衣壳冷冻电子显微镜显示独特的T = 16二十面体结构特征的疱疹病毒，虽然检查的制剂缺乏五邻体。OsHV-1的基因组结构与某些哺乳动物疱疹病毒（包括单纯疱疹病毒和人巨细胞病毒）相似，由两个可逆的独特区域（U-L，167-8 kbp; U-S，3-4 kbp）组成，每个区域两侧都有反向重复序列（TRL/IRL，7-6 kbp; TRS/IRS，9-8 kbp），在IRL和IRS之间还有一个额外的独特序列（X，1.5 kbp）。从207 439 bp的基因组序列中预测的124个独特基因中，有38个属于12个相关基因家族，编码的产物除膜蛋白相关蛋白外，还与解旋酶、凋亡抑制因子、脱氧尿苷三磷酸酶和环指蛋白有关。其中三个家族的八个基因似乎是片段化的。不属于这些家族的其他基因被预测编码DNA聚合酶、核糖核苷酸还原酶的两个亚基、解旋酶、引发酶、末端酶的ATP酶亚基、RecB样蛋白、其他RING样蛋白、离子通道和其他几种膜相关蛋白。序列比较表明，OsHV-1与两类脊椎动物疱疹病毒（与哺乳动物、鸟类和爬行动物相关的疱疹病毒，以及与硬骨鱼和两栖动物相关的疱疹病毒）的关系最好。因此OsHV-1代表疱疹病毒的第三大类。
Ostreid herpesvirus 1 (OsHV-1) is the only member of the Herpesviridae that has an invertebrate host and is associated with sporadic mortality in the Pacific oyster (Crassostrea gigas) and other bivalve species. Cryo-electron microscopy of purified capsids revealed the distinctive T = 16 icosahedral structure characteristic of herpesviruses, although the preparations examined lacked pentons. The gross genome organization of OsHV-1 was similar to that of certain mammalian herpesviruses (including herpes simplex virus and human cytomegalovirus), consisting of two invertible unique regions (U-L, 167-8 kbp; U-S, 3-4 kbp) each flanked by inverted repeats (TRL/IRL, 7-6 kbp; TRS/IRS, 9-8 kbp), with an additional unique sequence (X, 1.5 kbp) between IRL and IRS. Of the 124 unique genes predicted from the 207 439 bp genome sequence, 38 were members of 12 families of related genes and encoded products related to helicases, inhibitors of apoptosis, deoxyuridine triphosphatase and RING-finger proteins, in addition to me m bran e-associated proteins. Eight genes in three of the families appeared to be fragmented. Other genes that did not belong to the families were predicted to encode DNA polymerase, the two subunits of ribonucleotide reductase, a helicase, a primase, the ATPase subunit of terminase, a RecB-like protein, additional RING-like proteins, an ion channel and several other membrane-associated proteins. Sequence comparisons showed that OsHV-1 is at best tenuously related to the two classes of vertebrate herpesviruses (those associated with mammals, birds and reptiles, and those associated with bony fish and amphibians). OsHV-1 thus represents a third major class of the herpesviruses.