New bone formation enhanced by ADSCs overexpressing hRunx2 during mandibular distraction osteogenesis in osteoporotic rabbits
New bone formation enhanced by ADSCs overexpressing hRunx2 during mandibular distraction osteogenesis in osteoporotic rabbits
复制标题
DOI：
10.1002/jor.22590
复制
发表时间：
2014-05
影响因子：
2.8
通讯作者：
Jing-Jing Sun-Jing;Xiao-hui Zheng;Li-ya Wang;Lei Liu;W. Jing;Yunfeng Lin;W. Tian;Wei Tang;J. Long
中科院分区：
文献类型：
--
作者：
Jing-Jing Sun-Jing;Xiao-hui Zheng;Li-ya Wang;Lei Liu;W. Jing;Yunfeng Lin;W. Tian;Wei Tang;J. Long
在老年骨质疏松患者的牵张成骨（DO）过程中促进新骨形成仍然是一个挑战。在这项研究中，我们研究了使用局部Runt相关基因2的基因治疗对兔下颌骨发育不良DO期间新骨形成的影响。首先，我们成功地建立了一个下颌骨肥大的动物模型。第二，在皮质切除术后，将实验兔的右下颌骨牵张。A2组和B2组兔牵张间隙分别注射Adv‐ hRunx 2 ‐GFP转染的脂肪源性基质细胞（ADSCs）和Adv‐GFP转染的ADSCs。C2组（去卵巢对照组）和D2组（假手术对照组）注射生理盐水。在巩固期第3、6和9周，通过平片X线检查、显微计算机断层扫描、组织学检查和生物力学测试分析牵引间隙中的新一代骨组织。上述检查结果显示，B2、C2组未见理想新骨形成，A2、D2组有明显理想新骨形成。结果表明，使用rhRunx 2修饰的ADSCs的基因治疗促进了骨质疏松性下颌骨DO期间的新骨形成，并有效地补偿了全身性骨质疏松对新骨形成的不利影响。© 2014骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 32：709-720，2014.
Promoting new bone formation during distraction osteogenesis (DO) in elderly patients with osteoporosis is still a challenge. In this study, we investigated the effect of gene therapy using local Runt‐related gene 2 on new bone formation during osteoporotic mandibular DO in rabbits. First, we successfully established a mandibular osteoporotic animal model by ovariectomizing rabbits. Second, the right mandibles of the osteoporotic rabbits were distracted after corticotomy. The distraction gap of the rabbits in Group A2 and B2 were injected with Adv‐hRunx2‐GFP‐transfected adipose‐derived stromal cells (ADSCs) and Adv‐GFP‐transfected ADSCs, respectively. Rabbits in Groups C2 (ovariectomized control) and D2 (sham surgery control) were injected with physiologic saline. New‐generation bone tissue in the distraction gap was analyzed via plain radiographic examinations, micro‐computed tomography, histological examinations, and biomechanical testing at weeks 3, 6, and 9 of the consolidation period. Results of above examinations showed that no ideal new bone formation was observed in Groups B2 and C2, but obvious ideal new bone formation was observed in Group A2 and D2. The results suggested that gene therapy using rhRunx2‐modified ADSCs promoted new bone formation during osteoporotic mandibular DO and effectively compensated for the detrimental effects of systemic osteoporosis on new bone formation. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:709–720, 2014.