Elevated Expression of Bmi1 in Hepatocellular Carcinoma with Bile Duct Tumor Thrombi
Elevated Expression of Bmi1 in Hepatocellular Carcinoma with Bile Duct Tumor Thrombi
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Bmi1在肝细胞癌合并胆管癌栓中的表达升高
DOI：
10.5754/hge.12244
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发表时间：
2013-11-01
影响因子：
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通讯作者：
Liu, Chao
中科院分区：
文献类型：
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作者：
Zhang, Rui;Xu, Lei-Bo;Liu, Chao
背景/目的：肝细胞癌合并胆管癌栓(BDTT)是一种罕见事件。据报道，Bmi1基因在癌症的发生和发展中起着重要作用。本研究探讨Bmi1在BDTT肝细胞癌中的表达。方法：采用免疫组织化学方法分别检测BDTT患者(B+组)、肝细胞癌伴血管侵犯患者(V+组)及肝细胞癌胆管合并症患者(C+组)中Bmi1的表达。B+组13例，V+组10例，C+组6例，采用实时定量聚合酶链式反应检测新鲜标本中Bmi1基因的表达水平。结果：Bmi1免疫组织化学染色结果显示，B+组Bmi1表达明显高于C+组和V+组。实时定量聚合酶链式反应显示B+组Bmi1基因表达水平显著高于C+组和V+组。然而，有血管侵袭者和无血管侵袭者之间的Bmi1水平差异无统计学意义。结论：BMI1可能在肝细胞癌BDTT的发生发展中起重要作用。
Background/Aims: Hepatocellular carcinoma (HCC) with bile duct tumor thrombi (BDTT) is a rare event. The Bmi1 gene has been reported to play important roles in cancer initiation and progression. In this study, the expression of Bmi1 in HCC with BDTT was investigated. Methodology: The expression of Bmi1 was examined immunohistochemically in HCC patients with BDTT (B+ group), HCC patients with vascular invasion (V+ group) and combined hepatocellular carcinoma and cholangiocarcinoma(C+ group); respectively. Besides, the Bmi1 mRNA level was investigated by real time PCR in the fresh samples obtained from 13 cases in B+ group, 10 cases in V+ group, and 6 cases in C+ group, respectively. Results: Immunohistochemical staining for Bmi1 showed Bmi1 was highly expressed in the B+ group in comparison with the C+ group and the V+ group, respectively. The Bmi1 mRNA level by real time PCR also showed that it was significantly up-regulated in B+ group compared with those of C+ group and V+ group, respectively. However, there was no statistically significant difference in Bmi1 levels between the subjects with vascular invasion and the subjects without vascular invasion. Conclusions: Bmi1 might play important roles in the development of BDTT in HCC.