Plant virus particles with various shapes as potential adjuvants
Plant virus particles with various shapes as potential adjuvants
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DOI：
10.1038/s41598-020-67023-4
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发表时间：
2020-06-25
影响因子：
4.6
通讯作者：
Karpova, Olga V.
中科院分区：
文献类型：
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作者：
Evtushenko, Ekaterina A.;Ryabchevskaya, Ekaterina M.;Karpova, Olga V.
植物病毒对哺乳动物是生物安全的，并且可以成功地用作载体/平台以在创建新型推定疫苗的过程中呈递外源表位。然而，越来越多的证据表明，植物病毒，其病毒样和结构修饰的颗粒也可能对不与其表面共价结合的抗原具有免疫增强作用。在这里，我们目前的数据佐剂性质的植物病毒与各种形状（烟草花叶病毒，TMV，马铃薯病毒X，PVX，花椰菜花叶病毒，CaMV，菜豆温和花叶病毒，BMMV）和结构修饰的TMV球形颗粒（SP）。我们已经分析了在具有植物病毒/SP的组合物中对单个模型抗原（卵清蛋白、OVA/鸡蛋溶菌酶、HEL）和对OVA/HEL的免疫应答的有效性，并且已经表明CaMV、TMV和SP可以有效地诱导对模型抗原的总IgG滴度。在分析对植物病毒/SP本身的免疫应答时获得了一些有趣的数据。对CaMV、BMMV和PVX诱导了较强的免疫力，而TMV和SP刺激了相当低的自身IgG滴度。我们的研究结果为植物病毒的免疫增强特性提供了新的见解，并可用于设计基于植物病毒的佐剂。
Plant viruses are biologically safe for mammals and can be successfully used as a carrier/platform to present foreign epitopes in the course of creating novel putative vaccines. However, there is mounting evidence that plant viruses, their virus-like and structurally modified particles may also have an immunopotentiating effect on antigens not bound with their surface covalently. Here, we present data on the adjuvant properties of plant viruses with various shapes (Tobacco mosaic virus, TMV; Potato virus X, PVX; Cauliflower mosaic virus, CaMV; Bean mild mosaic virus, BMMV) and structurally modified TMV spherical particles (SPs). We have analysed the effectiveness of immune response to individual model antigens (ovalbumin, OVA/hen egg lysozyme, HEL) and to OVA/HEL in compositions with plant viruses/SPs, and have shown that CaMV, TMV and SPs can effectively induce total IgG titers to model antigen. Some intriguing data were obtained when analysing the immune response to the plant viruses/SPs themselves. Strong immunity was induced to CaMV, BMMV and PVX, whereas TMV and SPs stimulated considerably lower self-IgG titers. Our results provide new insights into the immunopotentiating properties of plant viruses and can be useful in devising adjuvants based on plant viruses.