Does recharging dental restorative materials with fluoride influence biofilm formation?
Does recharging dental restorative materials with fluoride influence biofilm formation?
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DOI：
10.1016/j.dental.2019.07.019
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发表时间：
2019-10-01
期刊：
影响因子：
5
通讯作者：
Hahnel, Sebastian
中科院分区：
文献类型：
--
作者：
Ionescu, Andrei;Brambilla, Eugenio;Hahnel, Sebastian
目标.目的：探讨含氟牙体充填材料对生物膜形成的影响。将由高粘度玻璃离子粘固剂（HVGIC）、树脂改性玻璃离子粘固剂（RMGIC）和树脂基复合物（RBC）制成的样本随机分配到人工唾液中孵育一周（AS-1）、五周（AS-5）和五周（包括每天用1450 ppm NaF牙膏刷牙两次）（AS-5-刷），或在孵育五周后一次性暴露于5000 ppm NaF（AS-5-exp）。人牙釉质用作参照。表面粗糙度和从标本中释放的氟化物进行了测定;生物膜的形成使用单或多物种的微生物模型进行模拟，并采用基于MTT的方法和共聚焦激光扫描显微镜分析。与RMGIC和RBC相比，HVGIC上的单种生物膜形成显著减少。与AS-5相比，AS-5-刷和AS-5-exp组中用氟化物处理后，HVGIC和釉质上的含量也降低。这些效果在24小时后特别明显，在生物膜形成48小时后不太明显。在多物种微生物模型中，HVGIC的观察结果相似，而AS-5-刷和AS-5-exp组中观察到釉质生物膜形成显著减少。无论采用何种微生物学模型，氟化物处理对RMGIC和RBC均无显著影响。这些数据表明，生物膜形成的表面上的玻璃离聚物水泥和牙釉质可以相关地影响处理与氟化物。牙釉质可以作为氟化物的储存库，需要定期补充。(C)2019年牙科材料学院。爱思唯尔公司出版All rights reserved.
Objectives. To investigate the influence of recharging dental restorative materials with fluoride on biofilm formation.Methods. Specimens produced from a high-viscosity glass ionomer cement (HVGIC), a resin-modified glass ionomer cement (RMGIC), and a resin-based composite (RBC) were randomly allotted to incubation in artificial saliva either for one week (AS-1), for five weeks (AS-5), for five weeks including twice/day brushing with 1450 ppm NaF toothpaste (AS-5-brush), or one time exposition to 5000 ppm NaF after five weeks of incubation (AS-5-exp). Human enamel was used as reference. Surface roughness and the release of fluoride from the specimens was determined; biofilm formation was simulated using mono- or multispecies microbiological models and analysed employing an MTT-based approach and confocal laser-scanning microscopy.Results. Monospecies biofilm formation was significantly reduced on HVGIC in comparison to RMGIC and RBC. It was also reduced on HVGIC and enamel after treatment with fluoride in groups AS-5-brush and AS-5-exp in comparison to AS-5. These effects were particularly pronounced after 24 h, and less pronounced after 48 h of biofilm formation. In the multispecies microbiological model, similar observations were identified for HVGIC, while for enamel a significant reduction in biofilm formation was observed in groups AS-5-brush and AS-5-exp. No significant effect of fluoride treatments was identified for RMGIC and RBC, regardless of the microbiological model applied.Significance. These data indicate that biofilm formation on the surfaces of a glass ionomer cement and enamel can be relevantly influenced by treatment with fluoride. Enamel may serve as a fluoride reservoir which requires regular recharge. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.