Genome sequencing reveals unique mutations in characteristic metabolic pathways and the transfer of virulence genes between V. mimicus and V. cholerae.
Genome sequencing reveals unique mutations in characteristic metabolic pathways and the transfer of virulence genes between V. mimicus and V. cholerae.
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基因组测序揭示了拟态弧菌和霍乱弧菌特征代谢途径的独特突变以及毒力基因的转移。
DOI：
10.1371/journal.pone.0021299
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kan B
中科院分区：
文献类型：
--
作者：
Wang D;Wang H;Zhou Y;Zhang Q;Zhang F;Du P;Wang S;Chen C;Kan B
拟态弧菌（Vibrio mimicus）是与霍乱弧菌最相似的一种，是一种存在于自然环境中的微生物，有时会导致人类腹泻和内部感染。它与霍乱弧菌表现出相似的表型，但在某些生化特性上有所不同。拟态弧菌和霍乱弧菌之间生化代谢差异的分子机制目前尚不清楚。已发现几种拟态弧菌分离株携带霍乱毒素基因（ctxAB），并在人类中引起霍乱样腹泻。在此，对携带完整CTX元件的拟态弧菌分离株SX-4的基因组进行测序和注释。其基因组的分析，连同其他弧菌物种，揭示了广泛的差异内弧菌科。在拟态弧菌菌株中发现了涉及用于区分拟态弧菌和霍乱弧菌的三种生化代谢途径的基因簇的共同突变。我们还构建了详细的基因组结构和进化图的一般类型的基因组漂移与致病性状的多糖，CTX元件和毒素共调节菌毛（TCP）基因簇。总体而言，携带霍乱毒素基因的拟态弧菌菌株的全基因组测序为理解弧菌属物种之间的基因组差异提供了详细信息。拟态弧菌与其最近的邻居霍乱弧菌具有大量不同的基因和核苷酸差异。在特征代谢途径中观察到的突变可能表明这些物种对不同生态位的不同适应，并且可能是由霍乱弧菌和拟态弧菌分歧之前的古老进化事件引起的。从霍乱弧菌的一个不常见的克隆，而不是第七次大流行菌株的毒力相关基因的水平转移，产生了携带霍乱毒素基因的致病性拟态弧菌菌株。
Vibrio mimicus, the species most similar to V. cholerae, is a microbe present in the natural environmental and sometimes causes diarrhea and internal infections in humans. It shows similar phenotypes to V. cholerae but differs in some biochemical characteristics. The molecular mechanisms underlying the differences in biochemical metabolism between V. mimicus and V. cholerae are currently unclear. Several V. mimicus isolates have been found that carry cholera toxin genes (ctxAB) and cause cholera-like diarrhea in humans. Here, the genome of the V. mimicus isolate SX-4, which carries an intact CTX element, was sequenced and annotated. Analysis of its genome, together with those of other Vibrio species, revealed extensive differences within the Vibrionaceae. Common mutations in gene clusters involved in three biochemical metabolism pathways that are used for discrimination between V. mimicus and V. cholerae were found in V. mimicus strains. We also constructed detailed genomic structures and evolution maps for the general types of genomic drift associated with pathogenic characters in polysaccharides, CTX elements and toxin co-regulated pilus (TCP) gene clusters. Overall, the whole-genome sequencing of the V. mimicus strain carrying the cholera toxin gene provides detailed information for understanding genomic differences among Vibrio spp. V. mimicus has a large number of diverse gene and nucleotide differences from its nearest neighbor, V. cholerae. The observed mutations in the characteristic metabolism pathways may indicate different adaptations to different niches for these species and may be caused by ancient events in evolution before the divergence of V. cholerae and V. mimicus. Horizontal transfers of virulence-related genes from an uncommon clone of V. cholerae, rather than the seventh pandemic strains, have generated the pathogenic V. mimicus strain carrying cholera toxin genes.
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DOI：
10.1073/pnas.1013825107
发表时间：
2010-12-07
影响因子：
11.1
作者：
Hasan, Nur A.;Grim, Christopher J.;Colwell, Rita R.
通讯作者：
Colwell, Rita R.
影响因子：
3.1
作者：
Faruque, SM;Rahman, MM;Mekalanos, JJ
通讯作者：
Mekalanos, JJ
影响因子：
64.5
作者：
Lee, SH;Hava, DL;Camilli, A
通讯作者：
Camilli, A
DOI：
10.1016/s0925-4439(97)00008-2
发表时间：
1997-04-12
影响因子：
6.2
作者：
Kim, GT;Lee, JY;Kong, IS
通讯作者：
Kong, IS
影响因子：
3.2
作者：
Mukhopadhyay, AK;Chakraborty, S;Berg, DE
通讯作者：
Berg, DE