Anti-inflammatory Effects of Limonene from Yuzu (Citrus junos Tanaka) Essential Oil on Eosinophils
Anti-inflammatory Effects of Limonene from Yuzu (Citrus junos Tanaka) Essential Oil on Eosinophils
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DOI：
10.1111/j.1750-3841.2010.01541.x
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发表时间：
2010-04-01
影响因子：
3.9
通讯作者：
Suganuma, Narufumi
中科院分区：
文献类型：
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作者：
Hirota, Ryoji;Roger, Ngatu Nlandu;Suganuma, Narufumi
鱼子(田中橘子)在日本被用作一种传统药物。我们研究了榆树皮中的柠檬烯对人嗜酸性粒细胞白血病HL-60克隆15细胞的体外抗炎作用。为了检测柠檬烯对细胞的抗炎作用，我们检测了细胞内活性氧(ROS)、单核细胞趋化蛋白-1(MCP-1)、核因子(NF)kappaB和p38丝裂原活化蛋白激酶(MAPK)的水平。我们发现低浓度的柠檬烯(7.34 mmol/L)抑制嗜酸性粒细胞趋化因子刺激的HL-60克隆15细胞ROS的产生。14.68 mmol/L浓度的柠檬烯通过激活核因子-kappa B抑制单核细胞趋化蛋白-1的产生，与添加蛋白酶体抑制剂MG132的作用相当。此外，与加入SB203580类似，它以p38MAPK依赖的方式抑制细胞的趋化作用。这些结果表明，柠檬烯可能通过抑制细胞因子、ROS的产生和抑制嗜酸性粒细胞的迁移而具有潜在的抗炎作用。
Yuzu (Citrus junos Tanaka) has been used as a traditional medicine in Japan. We investigated in vitro anti-inflammatory effects of limonene from yuzu peel on human eosinophilic leukemia HL-60 clone 15 cells. To examine anti-inflammatory effects of limonene on the cells, we measured the level of reactive oxygen species (ROS), monocyte chemoattractant protein-1 (MCP-1), nuclear factor (NF) kappa B, and p38 mitogen-activated protein kinase (MAPK). We found that low concentration of limonene (7.34 mmol/L) inhibited the production of ROS for eotaxin-stimulated HL-60 clone 15 cells. 14.68 mmol/L concentration of limonene diminished MCP-1 production via NF-kappa B activation comparable to the addition of the proteasomal inhibitor MG132. In addition, it inhibited cell chemotaxis in a p38 MAPK dependent manner similar to the adding of SB203580. These results suggest that limonene may have potential anti-inflammatory efficacy for the treatment of bronchial asthma by inhibiting cytokines, ROS production, and inactivating eosinophil migration.