Identification of phytotoxic metabolites released from Rehmannia glutinosa suggest their importance in the formation of its replant problem
Identification of phytotoxic metabolites released from Rehmannia glutinosa suggest their importance in the formation of its replant problem
复制标题
对地黄释放的植物毒性代谢物的鉴定表明它们在其重新种植问题的形成中的重要性
DOI：
10.1007/s11104-019-04136-4
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发表时间：
2019-08-01
期刊：
影响因子：
4.9
通讯作者：
Zhang, Zhongyi
中科院分区：
文献类型：
--
作者：
Zhang, Bao;Weston, Paul A.;Zhang, Zhongyi
背景与目的地黄药用块茎的再植问题严重制约了地黄药用块茎的产量和质量。然而，与该问题相关的潜在化感物质的起源和鉴定尚未得到很好的描述。为此，我们开展了一系列的相关研究，目的是鉴定谷草霉释放的主要植物毒性代谢物。收获并提取谷氨酰胺残留物用于代谢分析和生物测定定向鉴定。采用高压液相色谱-四极杆/飞行时间质谱联用技术分析了地黄叶片、块茎和根部周围土壤中的代谢物。采用皮氏培养皿滤纸和改良的帕克生物测定法对植物残体和候选化感物质的植物毒性进行了评价。结果利用非靶向代谢谱结合已知标准，鉴定/注释了17种地黄草特异性代谢物。结果表明，一种环烯醚萜苷和几种苯乙醇苷是主要的化感物质。结论植物和根际生物活性代谢物的鉴定增加了我们对地黄植物化学的认识。进一步评价这些生物活性化合物的土壤持久性和生态功能对确定地黄再植问题的具体机制具有重要意义。
Background and aimsThe yield and quality of medicinal tubers of Rehmannia glutinosa are seriously restricted by replanting problems. However, the origin and identification of the potential allelochemicals associated with this issue have not been well described. Therefore, in a series of related studies, we aimed to identify the key phytotoxic metabolites released from R. glutinosa.MethodsR. glutinosa residues were harvested and extracted for metabolic profiling and bioassay-directed identification. Metabolites in the leaf, tuber, and soil surrounding R. glutinosa roots were profiled using high pressure liquid chromatography coupled to a quadrupole/time of flight mass spectormeter. A Petri dish-filter paper and modified Parker bioassay were used to evaluate the phytotoxicity of plant residues and candidate allelochemicals.ResultsSeventeen metabolites specific to R. glutinosa were identified/annotated using non-targeted metabolic profiling combined with known standards. Assay results suggested an iridoid glycoside and several phenylethanoid glycosides as key allelochemicals originating from R. glutinosa.ConclusionIdentification of bioactive metabolites in planta and in the rhizosphere has increased our understanding of the phytochemistry of R. glutinosa. Further evaluation of the soil persistence and ecological functions of these bioactive compounds will be important for determination of the specific mechanisms associated with R. glutinosa replanting issues.