EXPRESSION OF BASEMENT-MEMBRANE PROTEINS AND INTERSTITIAL COLLAGENS IN DERMAL PAPILLAE OF HUMAN HAIR-FOLLICLES
EXPRESSION OF BASEMENT-MEMBRANE PROTEINS AND INTERSTITIAL COLLAGENS IN DERMAL PAPILLAE OF HUMAN HAIR-FOLLICLES
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DOI：
10.1111/1523-1747.ep12515907
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发表时间：
1991-01-01
影响因子：
6.5
通讯作者：
RANDALL, VA
中科院分区：
文献类型：
--
作者：
MESSENGER, AG;ELLIOTT, K;RANDALL, VA
用免疫组织化学方法研究了人毛囊间充质中基底膜分子和间质胶原的表达。 在整个毛发周期中，在真皮鞘和真皮乳头中发现了I型和III型胶原。 层粘连蛋白和IV型胶原蛋白表达于外根鞘基底膜和生长期和退化期毛囊毛乳头的细胞外基质中。 在休止期毛囊中，毛乳头细胞外基质的体积大大减少，毛乳头细胞层粘连蛋白和IV型胶原蛋白的轮廓染色仍然明显。 大疱性类天疱疮抗原的染色也见于外根鞘基底膜延伸到毛球的下尖。 在生长期毛囊中，在毛球上皮和毛乳头之间的界面处没有大疱性类天疱疮抗原染色，毛乳头内也没有染色。 然而，大疱性类天疱疮抗原的线性染色在退化期和休止期毛囊上皮周围变得连续。 从人真皮乳头培养的细胞也针对间质胶原、IV型胶原和层粘连蛋白染色。 然而，当培养的真皮成纤维细胞用相同的抗体染色时，获得了类似的结果。 人毛乳头中基底膜蛋白的表达与其他哺乳动物毛囊中的表达相似，表明这与毛乳头功能有关。 培养的毛乳头细胞表达与组织切片中所见相似的间质胶原和基底膜蛋白，但这一发现并不特异于毛乳头细胞。
The expression of basement membrane molecules and interstitial collagens in human hair follicle mesenchyme was studied by immunohistochemical staining of tissue sections and of cells cultured from dermal papillae. Type I and type III collagens were found in the dermal sheath and in the dermal papilla throughout the hair cycle. Laminin and type IV collagen were expressed at the outer root sheath basement membrane and in the extracellular matrix of the dermal papilla of anagen and catagen follicles. In telogen follicles, where the volume of the dermal papilla extracellular matrix is much reduced, outline staining of dermal papilla cells for laminin and type IV collagen was still apparent. Staining for bullous pemphigoid antigen was also seen at the outer root sheath basement membrane extending to the lower tip of the hair bulb. In anagen follicles, there was no staining for bullous pemphigoid antigen at the interface between hair bulb epithelium and the dermal papilla and no staining within the dermal papilla. However, linear staining for bullous pemphigoid antigen became continuous around hair follicle epithelium during catagen and telogen. Cells cultured from human dermal papillae also stained for interstitial collagens, type IV collagen and laminin. However, similar results were obtained when cultured dermal fibroblasts were stained with the same antibodies. The expression of basement membrane proteins in human dermal papillae resembles that seen in follicles from other mammalian species and suggests that this is relevant to dermal papilla function. Cultured dermal papilla cells express a similar pattern of interstitial collagens and basement membrane proteins to those seen in tissue sections but this finding is not specific to dermal papilla cells.