Inhibitory effects of thunberginols A and B isolated from Hydrangeae Dulcis Folium on mRNA expression of cytokines and on activation of activator protein-1 in RBL-2H3 cells
Inhibitory effects of thunberginols A and B isolated from Hydrangeae Dulcis Folium on mRNA expression of cytokines and on activation of activator protein-1 in RBL-2H3 cells
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DOI：
10.1016/j.phymed.2007.09.010
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发表时间：
2008-03-01
期刊：
影响因子：
7.9
通讯作者：
Yoshikawa, Masayuki
中科院分区：
文献类型：
--
作者：
Matsuda, Hisashi;Wang, Qiong;Yoshikawa, Masayuki
以前，从加工过的八仙花叶中提取了thunberginol A和B。结果表明，在RBL-2H3细胞中，白绣球（Hydrangea dulcis folium）能显著抑制抗原和钙离子载体A23187引起的细胞脱颗粒以及抗原引起的肿瘤坏死因子（tumor necrosis factor，TNF）-α和白细胞介素（interleukin，IL）-4释放。在本研究中，我们研究了Thunberginol B对几种细胞因子[IL-2，3，4和13，TNF-α和粒细胞/巨噬细胞集落刺激因子（GM-CSF）] mRNA表达的影响，以及Thunberginol A和B对RBL-2H3细胞中由c-jun和c-fos组成的激活蛋白（AP）-1的影响，AP-1是细胞因子mRNA表达所必需的。Thunberginol B抑制所有细胞因子的上调基因，Thunberginol A和B（30 μ M）抑制c-jun的磷酸化和c-fos mRNA的表达以及细胞外信号调节激酶（ERK 1/2）的磷酸化。此外，基因表达谱由thunberginol B是类似的，由木樨草素，一种天然黄酮具有强大的抗过敏作用。(c)2007年爱思唯尔有限公司。All rights reserved.
Previously, thunberginols A and B from the processed leaves of Hydrangeae macrophylla var. thunbergii (Hydrangea dulcis folium) substantially inhibited the degranulation caused by antigen and calcium ionophore A23187, and the release of tumor necrosis factor (TNF)-alpha and interleukin (IL)-4 by antigen in RBL-2H3 cells. In the present study, we examined the effect of thunberginol B on the expression of mRNA of several cytokines [ILs-2, 3, 4 and 13, TNF-alpha and granulocyte/macrophage-colony stimulating factor (GM-CSF)] and effects of thunberginols A and B on activator protein (AP)-1 composed of c-jun and c-fos, which is essential for the expression of the cytokine mRNA, in RBL-2H3 cells. Thunberginol B inhibited up-regulated genes of all cytokines, and thunberginols A and B (30 mu M) inhibited the phosphorylation of c-jun and expression of c-fos mRNA and phosphorylation of extracellular signal-regulated kinases (ERK1/2). In addition, the profile of gene expression by thunberginol B was similar to that by luteolin, a natural flavone with a potent anti-allergic effect. (c) 2007 Elsevier GmbH. All rights reserved.