gamma-Interferon stimulates production of 1,25-dihydroxyvitamin D3 by normal human macrophages.
gamma-Interferon stimulates production of 1,25-dihydroxyvitamin D3 by normal human macrophages.
复制标题
γ-干扰素刺激正常人巨噬细胞产生 1,25-二羟基维生素 D3。
DOI：
10.1016/s0006-291x(85)80202-3
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发表时间：
1985
影响因子：
3.1
通讯作者：
Norman,AW
中科院分区：
文献类型：
--
作者：
Koeffler,HP;Reichel,H;Bishop,JE;Norman,AW
我们首次发现正常人肺泡巨噬细胞在重组γ-干扰素(γ-干扰素)存在下培养时，其产生[~3H]-1，25(OH)2D3的基础速率显著增加。[~3H]-25(OH)_2D_3转化为[~3H]-1，25(OH)_2D_3的速率与剂量呈线性关系。在γ-干扰素作用1天后，PAM产生1，25(OH)2D3的量最大。这一最高高原水平持续了至少五天。从PAM中得到的1，25(OH)2D3与化学合成的1，25(OH)2D3在四种不同的高效液相色谱系统中精确地共迁移，验证了从PAM中得到的1，25(OH)2D3的真实性。
We show for the first time that normal human pulmonary alveolar macrophages (PAM) markedly enhance their basal rate of the production of [3H]--1,25(OH)2D3when cultured in the presence of recombinant γ-interferon (γ-IFN). The rate of conversion of [3H]-25(OH)D3to [3H]-1,25(OH)2D3was dose-dependent in a linear fashion. A maximal production of 1,25(OH)2D3by PAM occured after exposure of PAM to γ-IFN for one day. This maximum plateau-level was sustained for at least five days. The authenticity of the putative 1,25(OH)2D3obtained from PAM was tested by demonstrating the exact comigration of [3H]-1,25(OH)2D3with chemically synthesized 1,25(OH)2D3in four different HPLC-systems.