DEVELOPMENT OF A SENSITIVE 2-SITE IMMUNORADIOMETRIC ASSAY FOR PARATHYROID HORMONE-RELATED PEPTIDE - EVIDENCE FOR ELEVATED LEVELS IN PLASMA FROM PATIENTS WITH ADULT T-CELL LEUKEMIA/LYMPHOMA AND B-CELL LYMPHOMA
DEVELOPMENT OF A SENSITIVE 2-SITE IMMUNORADIOMETRIC ASSAY FOR PARATHYROID HORMONE-RELATED PEPTIDE - EVIDENCE FOR ELEVATED LEVELS IN PLASMA FROM PATIENTS WITH ADULT T-CELL LEUKEMIA/LYMPHOMA AND B-CELL LYMPHOMA
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DOI：
10.1210/jc.79.5.1322
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发表时间：
1994-11-01
影响因子：
5.8
通讯作者：
MATSUMOTO, T
中科院分区：
文献类型：
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作者：
IKEDA, K;OHNO, H;MATSUMOTO, T
我们开发了一种针对PTHrP的单克隆抗体（1-34）和针对PTHrP的多克隆抗体（50-83）的敏感免疫放射检测pth相关肽（PTHrP），以重组人PTHrP（1-87）为标准。免疫放射法检测限为0.5 pmol/L， 110名健康受试者血浆PTHrP（1-87）浓度为0.8 +/- 0.01 pmol/L，正常范围上限为1.1 pmol/L。在所有46例高钙血症的癌症患者中，循环PTHrP（1-87）浓度均升高，但在原发性甲状旁腺功能亢进、慢性肾功能衰竭或甲状旁腺功能减退的患者中没有升高。在2例肿瘤患者中，肿瘤切除后血钙水平的正常化与血浆PTHrP（1-87）浓度密切相关。高循环PTHrP（1-87）水平在13例高钙血症的成人t细胞白血病/淋巴瘤患者中有12例，在8例高钙血症的非霍奇金淋巴瘤（尤其是b细胞型）患者中有7例。这些结果表明PTHrP是导致高钙血症的主要体液因子，高钙血症通常与成人t细胞白血病/淋巴瘤和b细胞淋巴瘤相关。
We have developed a sensitive immunoradiometric assay for PTH-related peptide (PTHrP) using a monoclonal antibody against PTHrP(1-34) and a polyclonal antibody against PTHrP(50-83), with recombinant human PTHrP(1-87) as the standard. The detection limit of the immunoradiometric assay was 0.5 pmol/L, and plasma PTHrP(1-87) concentrations in 110 healthy subjects were 0.8 +/- 0.01 pmol/L, with the upper limit of the normal range being 1.1 pmol/L. Increased circulating PTHrP(1-87) concentrations were demonstrated in all 46 cancer patients with hypercalcemia, but not in patients with primary hyperparathyroidism, chronic renal failure, or hypoparathyroidism. Normalization of serum calcium levels after resection of tumors was shown to correlate well with that of plasma PTHrP(1-87) concentrations in 2 cancer patients. High circulating PTHrP(1-87) levels were also demonstrated in 12 out of 13 hypercalcemic patients with adult T-cell leukemia/lymphoma and in 7 out of 8 hypercalcemic patients with non-Hodgkin's lymphoma especially of B-cell type.These results suggest that PTHrP is a major humoral factor responsible for the hypercalcemia frequently associated with adult T-cell leukemia/lymphoma and also with B-cell lymphoma.