PREPARATION AND WATER RELAXATION PROPERTIES OF PROTEINS LABELED WITH PARAMAGNETIC METAL CHELATES
PREPARATION AND WATER RELAXATION PROPERTIES OF PROTEINS LABELED WITH PARAMAGNETIC METAL CHELATES
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DOI：
10.1016/0730-725x(85)90004-9
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
BRADY T J
中科院分区：
文献类型：
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作者：
LAUFFER R B;BRADY T J
采用双功能螯合剂法，用顺磁性Gd（III）和Mn（II）配合物标记牛血清白蛋白（BSA）和牛IG（IgG）。二乙烯三胺五乙酸（DTPA）和EDTA连接到几个游离氨基上的蛋白质使用这些配体的环酐形式。将金属离子Gd 3+和Mn 2+掺入螯合基团中产生高顺磁性蛋白质。在20 MHz下，蛋白质结合螯合物的水弛豫能力（或弛豫率）上级游离金属络合物。DTPA和EDTA缀合物之间弛豫率的差异很大程度上可以通过金属离子暴露于水的差异来解释。这种标记技术可用于制备血管内NMR造影剂（如顺磁性标记的人血清白蛋白）或靶向特异性试剂（标记的单克隆抗体或纤维蛋白原）。
The proteins bovine serum albumin (BSA) and bovine Ig (IgG) were labeled with paramagnetic Gd (III) and Mn (II) complexes using the bifunctional chelate approach. Diethylenetriaminepentaacetic acid (DTPA) and EDTA were attached to several free amino groups on the proteins using cyclic anhydride forms of these ligands. The incorporation of the metal ions Gd3+ and Mn2+ into the chelating groups yielded highly paramagnetic proteins. The water relaxation ability (or relaxivity) of the protein-bound chelates at 20 MHz was superior to that of the free metal complexes. Differences in relaxivity between the DTPA and EDTA conjugates could largely be accounted for by differences in the metal ion exposure to water. This labeling technique can be used in the preparation of intravascular NMR contrast agents (like paramagnetically-labeled human serum albumin) or target-specific agents (labeled monoclonal antibodies or fibrinogen).