Factors affecting the time of formation of the mouse blastocoele.
Factors affecting the time of formation of the mouse blastocoele.
复制标题
影响小鼠囊胚腔形成时间的因素。
DOI：
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复制
发表时间：
1977
期刊：
影响因子：
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通讯作者：
A. McLaren
中科院分区：
文献类型：
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作者：
R. Smith;A. McLaren
在体内发育的正常小鼠胚胎中，发现胚泡腔的首次出现与发育年龄（通过细胞数量判断）的关系比与实足年龄（即排卵后经过的时间）的关系更密切。当发育被体外培养减慢时，囊胚腔的形成被延迟。然而，细胞数量本身并不是一个关键因素，因为每个胚胎的细胞数量可以通过实验操作增加一倍或三倍或减半，而不会显著影响囊胚腔形成的时间。细胞松弛素B抑制一次细胞分裂，导致四倍体的实验表明，受精后细胞分裂的次数也不是关键。一个可能的作用是建议无论是核质比，或核或染色体分裂或DNA复制的数量，因为受精，所有这些都增加在卵裂。
In normal mouse embryos developing in vivo, the first appearance of the blastocyst cavity was found to be associated more closely with developmental age, judged by cell number, than with chronological age, i.e. elapsed time since ovulation. When development was slowed by in vitro culture, formation of the blastocoele was delayed. However, cell number itself was not a critical factor, since the number of cells per embryo could be doubled or tripled or halved by experimental manipulation without substantially affecting the timing of blastocoele formation. Experiments in which one cell division was suppressed with cytochalasin-B, leading to tetraploidy, showed that the number of cell divisions since fertilization was also not critical. A possible role is suggested either for nucleocytoplasmic ratio, or for the number of nuclear or chromosomal divisions or DNA replications since fertilization, all of which increase during cleavage.