Studying bacterial hydrophobicity and biofilm formation at liquid-liquid interfaces through interfacial rheology and pendant drop tensiometry
Studying bacterial hydrophobicity and biofilm formation at liquid-liquid interfaces through interfacial rheology and pendant drop tensiometry
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DOI：
10.1016/j.colsurfb.2014.02.023
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发表时间：
2014-05-01
影响因子：
5.8
通讯作者：
Fischer, P.
中科院分区：
文献类型：
--
作者：
Ruehs, P. A.;Boecker, L.;Fischer, P.
细菌对界面的吸附是生物膜形成的关键因素。了解生物膜形成和发育的一个主要限制是细菌细胞粘附到疏水界面的精确测量。在这项研究中，通过界面流变学和张力测量法监测了细菌在水-油界面的附着和生物膜的生长。5种模式细菌（恶臭假单胞菌ICT2442、恶臭假单胞菌W2、鼠伤寒沙门氏菌、大肠杆菌和枯草芽孢杆菌）分别在生长和不生长状态下吸附于油水界面。我们发现，通过界面流变学和张力学，我们可以观察到细菌附着的初始动力学和水-油界面瞬时生物膜的形成。电泳迁移率测量和细菌粘附碳氢化合物（BATH）测试进行表征选定的细菌。为了验证界面流变学和张力测量，我们使用共聚焦激光扫描显微镜和光学显微镜监测生物膜的形成。使用这种技术组合，我们能够观察到弹性和张力随时间的发展，从第一个细菌附着到生物膜的形成。(C) 2014 Elsevier B.V.版权所有
Bacterial adsorption to interfaces is a key factor in biofilm formation. One major limitation to understanding biofilm formation and development is the accurate measurement of bacterial cell adhesion to hydrophobic interfaces. With this study, bacterial attachment and biofilm growth over time at water-oil interface was monitored through interfacial rheology and tensiometry. Five model bacteria (Pseudomonas putida ICT2442, Pseudomonas putida W2, Salmonella typhimurium, Escherichia coli, and Bacillus subtilis) were allowed to adsorb at the water-oil interface either in their non-growing or growing state. We found that we were able to observe the initial kinetics of bacterial attachment and the transient biofilm formation at the water-oil interface through interfacial rheology and tensiometiy. Electrophoretic mobility measurements and bacterial adhesion to hydrocarbons (BATH) tests were performed to characterize the selected bacteria. To validate interfacial rheology and tensiometry measurements, we monitored biofilm formation utilizing both confocal laser scanning microscopy and light microscopy. Using this combination of techniques, we were able to observe the elasticity and tension development over time, from the first bacterial attachment up to biofilm formation. (C) 2014 Elsevier B.V. All rights reserved.