The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC36 and Saccharomyces cerevisiae Rfc3 subunits of replication factor C
The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC36 and Saccharomyces cerevisiae Rfc3 subunits of replication factor C
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DOI：
10.1007/s002940050514
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发表时间：
2000-03-01
期刊：
影响因子：
2.5
通讯作者：
MacNeill, SA
中科院分区：
文献类型：
--
作者：
Gray, FC;MacNeill, SA
在酿酒酵母和裂殖酵母中，复制因子C（RF-C）在染色体DNA复制和DNA复制检查点功能中起关键作用。在复制叉处，五亚基RF-C复合物的功能是将三聚体聚合酶辅助因子PCNA加载到DNA上。然后，PCNA充当滑动夹，将Pol δ拴在DNA上以最大化其持续合成能力。本文描述了S.粟酒裂殖酵母rfc 3（+）基因，编码S. cevevevisiae Rfc 3和人hRFC 36蛋白。1026 bp的rfc 3（+）ORF被5个内含子中断，大小从49到165 bp不等。剪接的ORF预计编码342个氨基酸的蛋白质，其在氨基酸序列水平上与S. cevevevisiae Rfc 3和人hRFC 36蛋白。正如我所料，S。粟酒裂殖酵母rfc 3（+）是一种必需基因，rfc 3 δ细胞的DNA复制有缺陷。rfc 3（+）功能的丧失可以通过异源表达S.酿酒酵母Rfc 3或人hRFC 36蛋白在S.粟酒
In the yeasts Saccharomyces cevevisiae and Schizosaccharomyces pombe replication factor C (RF-C) plays key roles both in chromosomal DNA replication and in DNA replication checkpoint function. At the replication fork, the five-subunit RF-C complex functions to load the trimeric polymerase accessory factor PCNA onto DNA. PCNA then acts as a sliding clamp tethering Pol delta to the DNA to maximise its processivity. Here we describe the cloning of the S. pombe rfc3(+) gene, encoding a homologue of the S. cevevisiae Rfc3 and human hRFC36 proteins. The 1026 bp rfc3(+) ORF is interrupted by five introns, ranging in size from 49 to 165 bp. The spliced ORF is predicted to encode a 342 amino-acid protein that is approximately 50% identical at the amino acid sequence level to the S. cevevisiae Rfc3 and human hRFC36 proteins. As expected, S. pombe rfc3(+) is an essential gene, with rfc3 Delta cells being defective for DNA replication. Loss of rfc3(+) function can be rescued by heterologous expression of either the S. cerevisiae Rfc3 or human hRFC36 proteins in S. pombe.