CHARACTERIZATION OF RECEPTORS FOR 1,25-DIHYDROXYVITAMIN-D3 IN THE HUMAN TESTIS
CHARACTERIZATION OF RECEPTORS FOR 1,25-DIHYDROXYVITAMIN-D3 IN THE HUMAN TESTIS
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DOI：
10.1016/0022-4731(90)90274-v
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发表时间：
1990-02-01
影响因子：
4.1
通讯作者：
GELLY, KJ
中科院分区：
文献类型：
--
作者：
HABIB, FK;MADDY, SQ;GELLY, KJ
1，25-二羟维生素D3的特异性高亲和力受体已在人类睾丸中得到证实。平均结合亲和力（Kd. ±. 1，25-二羟维生素D3受体的平均浓度（SD）为1.75 ± 0.01。0.32. times. 10-10 M，但结合能力低（平均NMax . ±.标准差= 0.53 ±。0.18 fmol/mg蛋白质）。结合是时间和温度依赖性的，在25 ℃下1小时后达到最大结合。C.虽然结合也发生在4和37 °。在25 ℃时发现更高和更快的结合。C.此外，配体和受体之间的结合是特异性的，因为只有未标记的1，25-二羟基维生素D3与标记的配体竞争。胰蛋白酶和加热可消除1，25-二羟维生素D3的结合。蔗糖密度梯度离心显示沉降系数为3.6S。
Specific high affinity receptors for 1,25-dihydroxyvitamin D3 have been demonstrated in the human testes. The mean binding affinity (Kd.+-. SD) of the receptor for 1,25-dihydroxyvitamin D3 was 1.75 .+-. 0.32 .times. 10-10 M but the binding capacity was low (mean NMax .+-. SD = 0.53 .+-. 0.18 fmol/mg protein). Binding was time- and temperature-dependent, with a maximum binding achieved after 1 h at 25.degree. C. Although binding also took place at 4 and 37.degree. C, higher and more rapid binding was found at 25.degree. C. Furthermore, the binding between the ligand and the receptor was specific since only unlabelled 1,25-dihydroxyvitamin D3 competed with the labelled ligand. Binding of 1,25-dihydroxyvitamin D3 was abolished by trypsin and heat. Sucrose density gradient centrifugation revealed a sedimentation coefficient of 3.6S.