Expression of bone morphogenetic proteins and rat distal-less homolog genes following rat femoral fracture
Expression of bone morphogenetic proteins and rat distal-less homolog genes following rat femoral fracture
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DOI：
10.1007/s007740050013
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发表时间：
2000-01-01
影响因子：
3.3
通讯作者：
Shimada, T
中科院分区：
文献类型：
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作者：
Yaoita, H;Orimo, H;Shimada, T
采用基于rt - pcr的技术，研究了大鼠股骨闭合性骨折后骨、痂和周围软组织中编码骨形态发生蛋白（BMP）、BMP - IA型受体（BMPR-1A）和大鼠无远端同源基因（rDlx）的表达。骨折前，编码BMP-5、BMP-6和bmp - 1a的基因在骨和周围软组织中均有表达，而BMP-2基因仅在骨中表达，BMP-7在两种组织中均未表达。这些基因的表达不受骨折的影响。编码BMP-4的基因也在骨折前在骨和周围软组织中表达。此外，虽然在骨中没有变化，但骨折后6小时周围软组织中BMP-4的表达增加了10倍。BMP-4表达的增加是短暂的，并在72h内恢复到骨折前的水平。骨折后骨中rDlx的表达也增加，但时间比BMP-4晚：rDlx的表达在48小时后升高，并持续30天或更长时间。骨折前后周围软组织均未见rDlx表达。这些发现表明BMP-4和rDlx在大鼠股骨骨折后选择性表达，也表明它们在骨折后骨愈合的早期阶段参与了骨痂的形成。
Expression of the genes encoding bone morphogenetic proteins (BMPs), BMP type IA receptor (BMPR-1A), and rat distal-less homolog (rDlx) was studied in bone, callus, and the surrounding soft tissue following rat femoral closed fracture, using RT-PCR-based techniques. Before fracture, the genes encoding BMP-5, BMP-6, and BMPR-1A were found to be expressed in both bone and the surrounding soft tissue, whereas the BMP-2 gene was expressed only in bone and BMP-7 was not expressed in either tissue. Expression of these genes was unaffected by fracture. The gene encoding BMP-4 was also expressed in both bone and the surrounding soft tissue before fracture. Moreover, although unchanged in bone, 6h after fracture BMP-4 expression was increased tenfold in the surrounding soft tissue. The increased BMP-4 expression was transient and returned to prefracture levels within 72h. Expression of rDlx was also increased in bone after fracture, but at later times than were observed with BMP-4: elevated rDlx expression was detected after 48h and persisted for 30 days or more. No expression of rDlx was observed in the surrounding soft tissue before or after fracture. These findings indicate that BMP-4 and rDlx are selectively expressed following femoral fracture in the rat, and also suggest that they are involved in the formation of the callus at an early point during the postfracture healing of bone.