Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype
Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype
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DOI：
10.1023/a:1025076805902
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发表时间：
2003-07-01
影响因子：
5.1
通讯作者：
Oliver, DJ
中科院分区：
文献类型：
--
作者：
Lin, M;Behal, R;Oliver, DJ
丙酮酸脱氢酶多酶复合物是植物质体中乙酰辅酶a形成的主要来源，由四个不同亚基e1α、e1β、E2和E3的多个拷贝组成。将T-DNA插入到拟南芥复合体的可塑性E2（二氢硫酰基乙酰转移酶）亚基plE2基因中，破坏了该基因的表达。由此产生的突变在杂合状态下没有明显的表型，但纯合突变是致命的。单倍体精子和卵子只含有被破坏的plE2基因功能，通常会形成一个突变的纯合子胚胎。这个胚胎只发育到早期阶段，然后发育停止，导致早期胚胎致死表型。虽然在35S、AtSERK1或napin启动子的控制下，该突变不能与plE2基因的cDNA互补，但可以利用内源性plE2启动子来驱动plE2 cDNA的表达。这证实了可塑丙酮酸脱氢酶复合体的本质及其在胚胎形成中的作用。
The pyruvate dehydrogenase multi-enzyme complex is the main source of acetyl-CoA formation in the plastids of plants and is composed of multiple copies of four different subunits, E1alpha, E1beta, E2, and E3. A T-DNA insertion into the gene for the plastidic E2 (dihydrolipoyl acetyltransferase) subunit, plE2, of the complex in Arabidopsis destroys the expression of that gene. The resulting mutation has no apparent phenotype in the heterozygous state, but the homozygous mutation is lethal. Haploid sperm and eggs that contain only the disrupted plE2 gene function normally resulting in the formation of an embryo that is homozygous for the mutation. This embryo only develops to an early stage before the development arrests resulting in an early embryo-lethal phenotype. While the mutation could not be complemented with the cDNA for the plE2 gene under control of the 35S, the AtSERK1, or the napin promoter, it could be complemented using the endogenous plE2 promoter to drive expression of the plE2 cDNA. This verifies the essential nature of the plastidic pyruvate dehydrogenase complex and its role in embryo formation.