Kernel principle component analysis applied to Raman spectra to differentiate drugs administered to rabbit cornea in blind study
Kernel principle component analysis applied to Raman spectra to differentiate drugs administered to rabbit cornea in blind study
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DOI：
10.1117/12.2546431
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发表时间：
2020-02
影响因子：
1.7
通讯作者：
Sean P. O’Connor;E. Gil;Mark A Keppler;M. Scully;V. Yakovlev
中科院分区：
文献类型：
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作者：
Sean P. O’Connor;E. Gil;Mark A Keppler;M. Scully;V. Yakovlev
扫描共焦拉曼光谱用于检测和鉴定兔角膜组织上局部使用的眼部药物。环孢菌素 A、二氟泼尼酯和多佐胺的拉曼光谱与使用未知量药物的兔角膜的拉曼光谱一起获得。然后使用核主成分分析 (KPCA) 来探索可以描述所获取的拉曼光谱集的变换。使用这种变换，我们观察到角膜光谱和环孢菌素 A 之间存在一些光谱相似性，而与多佐胺和二氟泼尼特几乎没有相似性。需要进一步调查以确定出现这些差异的原因。
Scanning confocal Raman spectroscopy was applied for detecting and identifying topically applied ocular pharmaceuticals on rabbit corneal tissue. Raman spectra for Cyclosporin A, Difluprednate, and Dorzolamide were acquired together with Raman spectra from rabbit corneas with an unknown amount of applied drug. Kernel principle component analysis (KPCA) was then used to explore a transform that can describe the acquired set of Raman spectra. Using this transform, we observe some spectral similarity between cornea spectra and Cyclosporin A, with little similarity to Dorzolamide and Difluprednate. Further investigation is needed to identify why these differences occur.