Cytotoxicity of Functionalized Polystyrene Latex Nanoparticles Toward Lactic Acid Bacteria, and Comparison with Model Microbes
Cytotoxicity of Functionalized Polystyrene Latex Nanoparticles Toward Lactic Acid Bacteria, and Comparison with Model Microbes
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功能化聚苯乙烯乳胶纳米粒子对乳酸菌的细胞毒性及其与模型微生物的比较
DOI：
10.1007/s11051-015-2922-8
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发表时间：
2015
影响因子：
2.5
通讯作者：
Y. Konishi
中科院分区：
文献类型：
--
作者：
T. Nomura;Y. Kuriyama;H. Tokumoto;Y. Konishi
研究了表面功能化聚苯乙烯乳胶 (PSL) 纳米颗粒 (NP)（标称直径：100 nm）对模型革兰氏阳性菌乳酸乳球菌 JCM 5805 的细胞毒性和胶体行为。几乎所有的L.暴露于带负电的 PSL NP 的乳酸细胞能够存活，因为细菌细胞表面带负电，因此 NP 很难粘附到细胞表面。相反，带正电荷的 PSL NP 粘附在 L 上。乳酸细胞表面但没有被截留在细胞内，随后发生细胞死亡。有毒NP暴露后的细菌生长曲线表明，NP毒性不影响特定的生长阶段，但确实影响滞后时间。这些结果表明，细胞因纳米颗粒粘附到细胞表面而导致细胞破裂而受损。最后，有毒、带正电荷的 PSL NP 对 L 的细胞毒性。将乳酸菌与模型革兰氏阴性细菌大肠杆菌和模型真核生物酿酒酵母进行比较。 NPs onL 的细胞毒性行为。乳酪和E.大肠杆菌相似，并且不取决于细菌表面结构，而是取决于环境离子强度。相反，原核细菌的细胞毒性高于模型真核生物。酿酒酵母。原核生物和真核生物对NP敏感性的差异是由于原核生物缺乏内吞途径造成的。
The cytotoxicity and colloidal behavior of surface-functionalized polystyrene latex (PSL) nanoparticles (NPs) (nominal diameter: 100 nm) toward a model gram positive bacteriumLactococcus lactisJCM 5805 were examined. Nearly all theL. lactiscells exposed to the negatively charged PSL NPs survived because the surface of the bacterial cell was charged negatively, and the NPs therefore hardly adhere to the cell surface. In contrast, the positively charged PSL NPs adhered to theL. lactiscell surface but were not entrapped within the cell, and cell death subsequently occurred. The bacterial growth curves after the toxic NP exposure suggested that NP toxicity did not affect the specific growth phase, but did affect lag time. These results indicated that the cells were damaged by the cell disruption that resulted from the adhesion of the NPs to the cell surface. Finally, the cytotoxicity of the toxic, positively charged PSL NPs towardL. lactiswas compared with that displayed toward a model gram negative bacteriumEscherichia coliand a model eukaryoteSaccharomyces cerevisiae. The cytotoxic behaviors of NPs onL. lactisandE. coliwere similar, and depended not on the bacterial surface structure, but rather the environmental ionic strength. In contrast, the cytotoxicity of the prokaryote bacteria was higher than that toward the model eukaryoteS. cerevisiae. The difference between the NP sensitivities of the prokaryote and eukaryote resulted from the prokaryote’s lack of an endocytotic pathway.