Grafting of polypeptides on solid substrates by initiation of N-carboxyanhydride polymerization by amino-terminated self-assembled monolayers
Grafting of polypeptides on solid substrates by initiation of N-carboxyanhydride polymerization by amino-terminated self-assembled monolayers
复制标题
通过氨基封端的自组装单层引发 N-羧酸酐聚合将多肽接枝到固体基质上
作者：
A. Heise;H. Menzel;H. Yim;M. Foster;R. H. Wieringa;A. Schouten;V. Erb;M. Stamm
在体硅衬底上制备了一系列不同组成的功能化（Br（CH 2）11 SiCl 3）和非功能化（CH 3（CH 2）10 SiCl 3）烷基三氯硅烷的混合自组装单分子膜。X射线光电子能谱测量显示，通过对这些单层进行原位修饰，溴末端基团转化为氨基末端基团。通过水接触角测量监测亲水性的变化，显示出随着表面上氨基含量的增加，接触角预期减小。这些底物用于在二氧六环中引发N-羧基氰基化以产生从表面接枝的α-螺旋多肽。所得聚合物层的厚度用椭圆偏振仪和X射线反射仪测量。傅立叶变换红外光谱分析证实接枝聚合物为α-螺旋构象。
A series of mixed self-assembled monolayers of functionalized (Br(CH2)11SiCl3) and unfunctionalized (CH3(CH2)10SiCl3) alkyltrichlorosilanes of different compositions have been prepared on bulk silicon substrates. By in situ modification of these monolayers the bromo end groups were transformed to amino end groups as shown by X-ray photoelectron spectroscopy measurements. The change of hydrophilicity was monitored by water contact angle measurements, showing the expected decrease of contact angles with increase amino group content on the surface. These substrates were used to initiate N-carboxyanhydrides in dioxane to yield α-helical polypeptides grafted from the surface. The thicknesses of the obtained polymer layers were measured with ellipsometry and X-ray reflectometry. Fourier transform infrared measurements confirm that the grafted polymers are in the α-helical conformation.