Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans.
Improved Biocompatibility of Amino-Functionalized Graphene Oxide in Caenorhabditis elegans.
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DOI：
10.1002/smll.201902699
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发表时间：
2019-10
期刊：
影响因子：
13.3
通讯作者：
Corvin Rive;G. Reina;Prerana Wagle;E. Treossi;V. Palermo;A. Bianco;Lucia Gemma-Delogu;Matthias Rieckher;B. Schumacher
中科院分区：
文献类型：
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作者：
Corvin Rive;G. Reina;Prerana Wagle;E. Treossi;V. Palermo;A. Bianco;Lucia Gemma-Delogu;Matthias Rieckher;B. Schumacher
氧化石墨烯 (GO) 在纳米医学的诊断和治疗应用中具有很高的前景，但据报道显示出免疫毒性，这凸显了开发具有改善的生物相容性的功能化 GO 的必要性。这项研究描述了 GO 和氨基功能化 GO (GONH2 ) 在急性或慢性暴露时在线虫发育和衰老过程中的不利影响。在整个秀丽隐杆线虫发育过程中进行慢性GO治疗会导致繁殖力下降和动物体型减小，而急性治疗不会导致任何可测量的生理下降。然而，RNA 测序数据表明，急性 GO 暴露会诱导先天免疫基因表达。 p38 MAP 激酶 PMK-1 是一种成熟的先天免疫主调节因子，可保护线虫免受慢性 GO 毒性，因为 pmk-1 突变体表现出组织功能降低和兼性胎生。在直接比较中，GONH2 暴露不会对野生型或 pmk-1 突变体造成有害影响，并且先天免疫反应也相当不明显。这项工作增强了氨基功能化 GO 在整个生物体中的生物相容性，强调了其作为生物医学纳米材料的潜力。
Graphene oxide (GO) holds high promise for diagnostic and therapeutic applications in nanomedicine but reportedly displays immunotoxicity, underlining the need for developing functionalized GO with improved biocompatibility. This study describes adverse effects of GO and amino-functionalized GO (GONH2 ) during Caenorhabditis elegans development and ageing upon acute or chronic exposure. Chronic GO treatment throughout the C. elegans development causes decreased fecundity and a reduction of animal size, while acute treatment does not lead to any measurable physiological decline. However, RNA-Sequencing data reveal that acute GO exposure induces innate immune gene expression. The p38 MAP kinase, PMK-1, which is a well-established master regulator of innate immunity, protects C. elegans from chronic GO toxicity, as pmk-1 mutants show reduced tissue-functionality and facultative vivipary. In a direct comparison, GONH2 exposure does not cause detrimental effects in the wild type or in pmk-1 mutants, and the innate immune response is considerably less pronounced. This work establishes enhanced biocompatibility of amino-functionalized GO in a whole-organism, emphasizing its potential as a biomedical nanomaterial.