Modeling electroporation in a single cell. II. Effects of ionic concentrations
Modeling electroporation in a single cell. II. Effects of ionic concentrations
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DOI：
10.1016/s0006-3495(99)76974-2
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Krassowska, W
中科院分区：
文献类型：
--
作者：
DeBruin, KA;Krassowska, W
这项研究通过明确地解释电穿孔电流的离子组成，扩展了先前开发的单个电池被外部电场电穿孔的模型。以前的非特定电穿孔电流模型预测跨膜电位V-m和孔密度N都是关于赤道对称的，在电池的两端都有相同的值。新的离子特定情况预测，V-m是对称的，与非特定情况下的轮廓几乎相同，但N具有严重的不对称性，细胞超极化一端的孔密度是去极化一端的两倍。这些模拟结果与实验观察到的标记分子在细胞超极化端的优先摄取一致，如文献所述。这项研究还调查了电穿孔单个细胞周围引起的细胞内离子浓度的变化。对于所有离子物种，膜附近的浓度变化很大，这可能解释了在大电击传递到可兴奋的细胞和组织后实验观察到的电干扰。
This study expands a previously developed model of a single cell electroporated by an external electric field by explicitly accounting for the ionic composition of the electroporation current. The previous model with non-specific electroporation current predicts that both the transmembrane potential V-m and the pore density N are symmetric about the equator, with the same values at either end of the cell. The new, ion-specific case predicts that V-m is symmetric and almost identical to the profile from the non-specific case, but N has a profound asymmetry with the pore density at the hyperpolarized end of the cell twice the value at the depolarized end. These modeling results agree with the experimentally observed preferential uptake of marker molecules at the hyperpolarized end of the cell as reported in the literature. This study also investigates the changes in intracellular ionic concentrations induced around an electroporated single cell. For all ion species, the concentrations near the membrane vary significantly, which may explain the electrical disturbances observed experimentally after large electric shocks are delivered to excitable cells and tissues.