Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis
Biocompatible Cantilevers for Mechanical Characterization of Zebrafish Embryos using Image Analysis
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DOI：
10.3390/s19071506
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
Yuji Tomizawa;Krishna Dixit;D. Daggett;K. Hoshino
中科院分区：
文献类型：
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作者：
Yuji Tomizawa;Krishna Dixit;D. Daggett;K. Hoshino
我们开发了一种力传感系统来连续评估微米级（几百微米到一毫米）活组织的机械弹性。传感是通过显微图像分析测量力敏感悬臂梁的挠度来实现的，而不需要电应变片。由生物相容性聚二甲基硅氧烷（PDMS）制成的悬臂由压电驱动器驱动，并作为一双筷子来测量样品的刚度。悬臂梁的尺寸很容易调整，以匹配斑马鱼样本的尺寸、范围和刚度。在本文中，我们通过测量斑马鱼胚胎在不同发育阶段的机械弹性来证明这种技术的多功能性。每小时测量一次斑马鱼胚胎的硬度，持续9 h。从实验结果中，我们成功地量化了斑马鱼胚胎在胚胎发育过程中的硬度变化。
We have developed a force sensing system to continuously evaluate the mechanical elasticity of micrometer-scale (a few hundred micrometers to a millimeter) live tissues. The sensing is achieved by measuring the deflection of force sensitive cantilevers through microscopic image analysis, which does not require electrical strain gauges. Cantilevers made of biocompatible polydimethylsiloxane (PDMS) were actuated by a piezoelectric actuator and functioned as a pair of chopsticks to measure the stiffness of the specimen. The dimensions of the cantilevers were easily adjusted to match the size, range, and stiffness of the zebrafish samples. In this paper, we demonstrated the versatility of this technique by measuring the mechanical elasticity of zebrafish embryos at different stages of development. The stiffness of zebrafish embryos was measured once per hour for 9 h. From the experimental results, we successfully quantified the stiffness change of zebrafish embryos during embryonic development.