Vitamin D deficiency decreases the expression of VDR and prohibitin in the lungs of mice with allergic airway inflammation.
Vitamin D deficiency decreases the expression of VDR and prohibitin in the lungs of mice with allergic airway inflammation.
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DOI：
10.1016/j.yexmp.2012.04.004
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发表时间：
2012-08
影响因子：
3.6
通讯作者：
Agrawal, Devendra K.
中科院分区：
文献类型：
--
作者：
Agrawal, Tanupriya;Gupta, Gaurav K.;Agrawal, Devendra K.
关键词：
Asthma is one of the most common chronic inflammatory diseases of the airways. Calcitriol exerts its action through Vitamin D receptor (VDR), which is a high affinity nuclear receptor. VDR is a transcription factor that alters the transcription of target genes which are involved in a wide spectrum of biological responses. Lower serum vitamin D levels are associated with airway hyperresponsiveness and increased asthma severity. Prohibitin is a ubiquitously expressed protein localized to the cell and mitochondrial membranes and the nucleus. HBSMCs were cultured and treated with calcitriol and/or TNF-α. The mRNA and protein expression of prohibitin and VDR were analyzed using qPCR and immunoblotting, respectively. In the in vivo studies, female BALB/c mice were fed with special vitamin D-deficient or 2,000 IU/kg of vitamin D-supplemented diet for 13 weeks. Mouse model of allergic airway inflammation was developed by OVA-sensitization and challenge. The expression pattern of TNF-α, prohibitin and VDR in the lung of OVA-sensitized mice was analyzed using immunofluorescence. Calcitriol significantly increased and TNF-α decreased the protein and mRNA expression of prohibitin and VDR in HBSMCs. There was significantly increased expression of TNF-α and decreased expression of VDR and prohibitin in the lung of vitamin D-deficient mouse model of allergic airway inflammation. These results suggest that under inflammatory conditions there is decreased expression of VDR resulting in decreased expression of prohibitin, which is a vitamin D target gene. Vitamin D deficiency causes increase in the expression of TNF-α, thereby increasing inflammation and decreases the expression of VDR and prohibitin. Supplementation with vitamin D reduces the levels of TNF-α, thereby increasing the expression of VDR and prohibitin that could be responsible for reducing allergic airway inflammation.
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DOI：
10.1165/rcmb.2009-0330oc
发表时间：
2011-03-01
影响因子：
6.4
作者：
Makinde, Toluwalope O.;Agrawal, Devendra K.
通讯作者：
Agrawal, Devendra K.
影响因子：
3.4
作者：
Hsieh, Sen-Yung;Shih, Tsung-Chieh;Lee, Ying-Shiung
通讯作者：
Lee, Ying-Shiung
影响因子：
4.8
作者：
Fusaro, G;Dasgupta, P;Chellappan, S
通讯作者：
Chellappan, S
DOI：
10.1016/j.bbamcr.2011.01.033
发表时间：
2011-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Theiss AL;Sitaraman SV
通讯作者：
Sitaraman SV
DOI：
10.1165/rcmb.2009-0241oc
发表时间：
2010-11-01
影响因子：
6.4
作者：
McGee, Halvor S.;Stallworth, Arthur L.;Agrawal, Devendra K.
通讯作者：
Agrawal, Devendra K.