Evaluation of a glassy carbon electrode modified by a bilayer lipid membrane with incorporated DNA.
Evaluation of a glassy carbon electrode modified by a bilayer lipid membrane with incorporated DNA.
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DOI：
10.1016/0039-9140(96)01881-4
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发表时间：
1996-07
期刊：
影响因子：
6.1
通讯作者：
C. Siontorou;A. M. Oliveira Brett;D. Nikolelis
中科院分区：
文献类型：
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作者：
C. Siontorou;A. M. Oliveira Brett;D. Nikolelis
本研究的目的是评价和表征一种由掺入单链脱氧核糖核酸（fss DNA）的双层脂质膜（BLM）修饰的玻璃碳（GC）电极。在脂质膜修饰的电极上，开发并测试了各种方法用于ss DNA的结合：差分脉冲伏安法（即鸟嘌呤和腺嘌呤残基的氧化）用于监测ss DNA在BLM修饰的GC电极上的结合。结果表明，脂质膜在电极的“介质交换”过程中增强了ss DNA的稳定性，并阻止其从电极表面扩散。与前两种技术相比，第三种方案被证明是最合适的，因为BLM的电极修饰和DNA吸附都发生在一个阶段，而且速度更快（因为不需要BLM稀释过程）；此外，在blm中实现了DNA的最大负载，这减少了大约10倍的DNA量，可以检测到电化学。使用传统的平面“自由悬浮”和自组装金属支撑的blm来原位监测这些膜中ss DNA的掺入。结果表明，与先前描述的技术相比，使用更温和的条件和更少量的DNA， ss DNA在脂质膜上的吸附（作为电极表面DNA结合的介质）可以更快地发生。
The objective of the present work was the evaluation and characterization of a glassy carbon (GC) electrode modified by a bilayer lipid membrane (BLM) with incorporated single-stranded deoxyribonucleic acid fss DNA). Various procedures were developed and tested for the incorporation of ss DNA at the electrode modified by the lipidic membrane: Differential pulse voltammetry (i.e. oxidation of guanine and adenine residues) was used to monitor the incorporation of ss DNA at the GC electrode modified by the BLM. The results have shown that the lipid membrane enhances the stability of ss DNA during a “medium-exchange” of the electrode and prohibits its diffusion from the electrode surface. The third scheme was proven to be the most appropriate as both electrode modification by the BLM and DNA adsorption occur in one stage and much faster (as no BLM thinning process is required) as compared to the former two techniques; furthermore, maximized loading of DNA in BLMs is achieved which reduces by ca. 10-fold the DNA amounts that can be detected electrochemically. Conventional planar “free-suspended” and self-assembled metal supported BLMs were used to monitor in situ the incorporation of ss DNA in these membranes. The results have shown that the adsorption of ss DNA at lipid membranes (as a medium for DNA incorporation on an electrode surface) can occur much faster, using milder conditions and smaller amounts of DNA than by previously described techniques.