Identification and partial characterization of Mycobacterium avium and Mycobacterium intracellulare by using DNA probes
Identification and partial characterization of Mycobacterium avium and Mycobacterium intracellulare by using DNA probes
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使用 DNA 探针对鸟分枝杆菌和胞内分枝杆菌进行鉴定和部分表征
DOI：
10.1128/jcm.27.5.994-997.1989
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发表时间：
1989
影响因子：
9.4
通讯作者：
Kenji Asano
中科院分区：
文献类型：
--
作者：
Hajime Saito;Haruaki Tomioka;'. Katsumasa;Sato;H. Tasaka;Michio Tsukamura;Fumiyuki Kuze;Kenji Asano
我们试图通过使用M.禽分枝杆菌或胞内分枝杆菌（MAC的Gen-Probe快速诊断系统; Gen-Probe，Inc.，加利福尼亚州圣地亚哥）。分析了病原菌的来源和药敏分布。该检测系统被证明是快速，灵敏，特异性和可靠的鉴定MAC和物种作为M。鸟或M。胞内的DNA探针检测表明，在公认的MAC血清型中，血清型1 ~ 6、8 ~ 11和21属于M。7和12 ~ 20只属于M.胞内的此外，通过DNA探针检测，发现M. avium和M.日本的细胞内分离株因MAC分离地区的不同而有所不同。M.日本东部的鸟类数量要高得多。在东海区和岛根区，M. avium和M.在人类疾病中显著的胞内分离物与来自土壤和房屋灰尘（自然来源）的分离物相关。In M.禽流感、人类疾病相关的分离株对利福平、链霉素和卡那霉素的耐药性比来自天然来源的分离株更强。然而，这种来源依赖性对M.胞内的在人类疾病相关MAC中，M.禽分枝杆菌对除喹诺酮类外的大多数药物的耐药性高于M.胞内分离物。
We attempted to identify the Mycobacterium avium complex (MAC) isolated in Japan by using DNA probes specific for M. avium or Mycobacterium intracellulare (Gen-Probe Rapid Diagnostic System for MAC; Gen-Probe, Inc., San Diego, Calif.). The source and drug susceptibility distributions were examined. This assay system proved to be rapid, sensitive, specific, and reliable for identification of MAC and of the species as either M. avium or M. intracellulare. The DNA probe test showed that of the generally accepted MAC serovars, serovars 1 to 6, 8 to 11, and 21 belonged to M. avium and 7 and 12 to 20 belonged to M. intracellulare. Moreover, with the DNA probe test we found that the distribution patterns of M. avium and M. intracellulare isolates in Japan differed depending on the district in which MAC was isolated. The ratio of M. avium was much higher in eastern Japan. In Tokai and Shimane districts, the ratio of M. avium and M. intracellulare isolates significant in human disease was related to that of isolates from soil and house dust (natural sources). In M. avium, human disease-associated isolates were more resistant to rifampin, streptomycin, and kanamycin than were isolates from natural sources. However, this source dependence was not evident for M. intracellulare. In human disease-associated MAC, M. avium isolates were more resistant to most agents, except for quinolones, than were M. intracellulare isolates.