Bottom-up assembly of RNA arrays and superstructures as potential parts in nanotechnology
Bottom-up assembly of RNA arrays and superstructures as potential parts in nanotechnology
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DOI：
10.1021/nl0494497
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发表时间：
2004-09-01
期刊：
影响因子：
10.8
通讯作者：
Guo, PX
中科院分区：
文献类型：
--
作者：
Shu, D;Moll, WD;Guo, PX
DNA和蛋白质作为纳米技术的一部分已经被广泛地审查了可行性，但另一种天然的构建模块RNA在很大程度上被忽视了。RNA可以被操纵以形成多种形状，从而为DNA纳米技术提供了适应性元素，DNA纳米技术主要基于双螺旋结构。细菌病毒phi 29的DNA包装马达包含六个DNA包装RNA（pRNA），它们通过环/环相互作用一起形成六聚体环。在这里，我们报告说，这种pRNA可以重新设计，形成各种结构和形状，包括双胞胎，四聚体，棒，三角形，和3D阵列的大小几微米，通过相互作用的编程螺旋区域和环。三维RNA阵列的形成需要一个确定的核苷酸数量的相互作用的螺旋和回文序列的扭曲。这样的阵列异常稳定，并且耐受广泛的温度、盐浓度和pH。
DNA and protein have been extensively scrutinized for feasibility as parts in nanotechnology, but another natural building block, RNA, has been largely ignored. RNA can be manipulated to form versatile shapes, thus providing an element of adaptability to DNA nanotechnology, which is predominantly based upon a double-helical structure. The DNA-packaging motor of bacterial virus phi29 contains six DNA-packaging RNAs (pRNA), which together form a hexameric ring via loop/loop interaction. Here we report that this pRNA can be redesigned to form a variety of structures and shapes, including twins, tetramers, rods, triangles, and 3D arrays several microns in size via interaction of programmed helical regions and loops. Three dimensional RNA array formation required a defined nucleotide number for twisting of the interactive helix and a palindromic sequence. Such arrays are unusually stable and resistant to a wide range of temperatures, salt concentrations, and pH.