DIFFERENTIATION OF T-CELL LYMPHOKINE GENE-EXPRESSION - THE INVITRO ACQUISITION OF T-CELL MEMORY
DIFFERENTIATION OF T-CELL LYMPHOKINE GENE-EXPRESSION - THE INVITRO ACQUISITION OF T-CELL MEMORY
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DOI：
10.1084/jem.173.1.25
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发表时间：
1991-01-01
影响因子：
15.3
通讯作者：
SMITH, KA
中科院分区：
文献类型：
--
作者：
EHLERS, S;SMITH, KA
设计了一个简单的体外实验系统来反映 T 细胞记忆反应的体内生成，以便可以在淋巴因子基因表达水平上检查 T 细胞分化。 新生儿和成人 T 细胞的比较显示，两个群体都表达白细胞介素 2 (IL-2) 及其受体的基因，但只有成人 T 细胞能够转录 IL-3、IL-4、IL-5、IL-6、干扰素-γ 和粒细胞/巨噬细胞集落刺激因子的 mRNA。 然而，新生T细胞可以在体外被诱导进行功能分化，从而获得表达成人T细胞特征的淋巴因子基因库的能力。 这些数据表明，通过体外初级刺激从新生儿血液中产生的 T 细胞在功能上与成人血液中可能已经经历了体内初级刺激的 T 细胞在功能上没有区别。 因此，我们建议术语“记忆细胞”适用于那些可以通过诱导效应淋巴因子基因表达的分化状态来识别的T细胞。
A simple in vitro experimental system was devised to reflect the in vivo generation of a T cell anamnestic response so that T cell differentiation could be examined at the level of lymphokine gene expression. Comparison of neonatal and adult T cells revealed that both populations expressed the genes for interleukin 2 (IL-2) and its receptor, but only adult T cells were capable of transcribing mRNAs for IL-3, IL-4, IL-5, IL-6, interferon-gamma, and granulocyte/macrophage colony-stimulating factor. However, neonatal T cells could be induced to undergo functional differentiation in vitro, thereby acquiring the capacity to express the lymphokine gene repertoire characteristic for adult T cells. These data suggest that the T cells generated from neonatal blood by a primary stimulation in vitro are functionally indistinguishable from the T cells in adult blood that presumably have undergone primary stimulation in vivo. Therefore, we propose that the term "memory cell" be applied to those T cells that can be identified by their differentiated state of inducible effector-lymphokine gene expression.