Turnip mosaic virus VPg interacts with Arabidopsis thaliana eIF(iso)4E and inhibits in vitro translation
Turnip mosaic virus VPg interacts with Arabidopsis thaliana eIF(iso)4E and inhibits in vitro translation
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DOI：
10.1016/j.biochi.2008.03.013
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发表时间：
2008-10-01
期刊：
影响因子：
3.9
通讯作者：
Natsuaki, Tomohide
中科院分区：
文献类型：
--
作者：
Miyoshi, Hiroshi;Okade, Hayato;Natsuaki, Tomohide
研究了芜菁花叶病毒（TuMV）基因组连接蛋白（VPg）与拟南芥真核起始因子（iso）4 E（eIF（iso）4 E）的相互作用，探讨了马铃薯Y病毒VPg对宿主细胞翻译起始的影响。亲和层析分析表明，VPg的62-70位氨基酸组成的区域对与eIF（iso）4 E的相互作用是重要的。体外翻译分析表明，VPg的加入显着抑制加帽RNA在eIF（iso）4 E-重构小麦胚芽提取物的翻译。该结果表明VPg通过与eIF（iso）4 E结合抑制帽依赖性翻译起始。VPg对小麦胚芽提取物体外翻译RNA的抑制作用不依赖于RNase活性。我们目前的研究结果可能表明，过量的VPg产生的在pH4.0阶段关闭帽依赖性的翻译起始在宿主细胞中通过抑制复合物的形成eIF（iso）4 E和细胞的mRNA。(C)2008年，Elsevier Masson SAS。All rights reserved.
The interaction between turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) and Arabidopsis thaliana eukaryotic initiation factor (iso)4E (eIF(iso)4E) was investigated to address the influence of potyviral VPg on host cellular translational initiation. Affinity chromatographic analysis showed that the region comprising amino acids 62-70 of VPg is important for the interaction with eIF(iso)4E. In vitro translation analysis showed that the addition of VPg significantly inhibited translation of capped RNA in eIF(iso)4E-reconstituted wheat germ extract. This result indicates that VPg inhibits cap-dependent translational initiation via binding to eIF(iso)4E. The inhibition by VPg of in vitro translation of RNA with wheat germ extract did not depend on RNase activity. Our present results may indicate that excess VPg produced at the encapsidation stage shuts off cap-dependent translational initiation in host cells by inhibiting complex formation between eIF(iso)4E and cellular mRNAs. (C) 2008 Elsevier Masson SAS. All rights reserved.