IF1 INHIBITION OF MITOCHONDRIAL F1-ATPASE IS CORRELATED TO ENTRAPMENT OF 4 ADENINE-NUCLEOTIDES OR GUANINE-NUCLEOTIDES INCLUDING AT LEAST ONE TRIPHOSPHATE
IF1 INHIBITION OF MITOCHONDRIAL F1-ATPASE IS CORRELATED TO ENTRAPMENT OF 4 ADENINE-NUCLEOTIDES OR GUANINE-NUCLEOTIDES INCLUDING AT LEAST ONE TRIPHOSPHATE
复制标题
DOI：
10.1016/s0006-291x(88)80429-7
复制
发表时间：
1988-05-16
影响因子：
3.1
通讯作者：
GAUTHERON, DC
中科院分区：
文献类型：
--
作者：
DIPIETRO, A;PENIN, F;GAUTHERON, DC
本文证明天然抑制剂蛋白 IF1 对 F1 ATP 酶活性的抑制与 F1 中三磷酸核苷酸的捕获有关。与用于促进 IF1 抑制的 Mg-[α-32P]GTP 或 Mg-[α-32P]ATP 预孵育后结合的核苷酸的完全平衡已在含有 0.7 mol 不可交换内源核苷酸的纯化 F1 上建立。 F1 中捕获了多达 4 mol 的标记鸟嘌呤或腺嘌呤核苷酸；这些核苷酸中至少一种是三磷酸。相反，在没有 IF1 的情况下，三磷酸核苷酸不会被显着保留，并且结合的二磷酸核苷酸主要是可交换的。
This paper demonstrates that the inhibition of F1 ATPase activity by the natural inhibitor protein IF1 is correlated to triphosphate nucleotide entrapment in F1. The complete balance of nucleotides bound after preincubation with Mg-[α-32P]GTP or Mg-[α-32P]ATP, used to promote IF1 inhibition, has been established on purified F1 containing 0.7 mol of non-exchangeable endogenous nucleotides. As many as 4 mol of labelled guanine- or adenine- nucleotides are trapped in F1; at least one of these nucleotides is a triphosphate. On the contrary, in the absence of IF1, no triphosphate nucleotide is significantly retained and the diphosphate nucleotides bound are mainly exchangeable.