Bovine viral diarrhoea virus antigen in foetal calf serum batches and consequences of such contamination for vaccine production
Bovine viral diarrhoea virus antigen in foetal calf serum batches and consequences of such contamination for vaccine production
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DOI：
10.1016/s1045-1056(03)00058-7
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发表时间：
2003-09-01
期刊：
影响因子：
1.7
通讯作者：
Goovaerts, D
中科院分区：
文献类型：
--
作者：
Makoschey, B;Keijsers, V;Goovaerts, D
描述了一种检测牛病毒性腹泻病毒（BVDV）污染的胎牛血清（FCS）的方案。采用细胞培养法和鼠疫病毒RNA检测相结合的方法，对7批FCS进行了检测。在其中四个批次中检测到传染性BVDV。其余一批中有一批含有相对较多的非传染性BVDV颗粒。该批样品以氢氧化铝和磷酸铝作为佐剂配制成实验性疫苗制剂。BVDV血清阴性的牛每隔4周注射2次。第二次应用后4周采集的血液样本BVDV特异性抗体呈阴性。我们的数据强调，BVDV RNA的检测不足以对FCS进行完整的风险评估。区分传染性和非传染性BVDV至关重要。这只能通过细胞培养方法来实现。(C) 2003国际生物制品协会。Elsevier Ltd.出版。版权所有。
A protocol to test foetal calf serum (FCS) for contamination with bovine viral diarrhoea virus (BVDV) is described. Following this protocol, which combines cell culture methods and detection of pestivirus RNA, seven batches of FCS were tested. Infectious BVDV was detected in four of those batches. One of the remaining batches contained a relatively high number of non-infectious BVDV particles. A sample of this batch was formulated with aluminium hydroxide and aluminium phosphate as adjuvant into an experimental vaccine preparation. This product was injected twice into BVDV seronegative cattle with a 4 week interval. Blood samples taken 4 weeks after the second application were negative for BVDV specific antibodies. Our data stress that detection of BVDV RNA is not sufficient for a complete risk assessment on FCS. Discrimination between infectious and non-infectious BVDV is essential. This can only be achieved by cell culture methods. (C) 2003 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.