Antinociceptive activity of extracts and secondary metabolites from wild growing and micropropagated plants of Renealmia alpinia.
Antinociceptive activity of extracts and secondary metabolites from wild growing and micropropagated plants of Renealmia alpinia.
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DOI：
10.1016/j.jep.2015.02.012
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发表时间：
2015-05-13
影响因子：
5.4
通讯作者：
Cutler SJ
中科院分区：
文献类型：
--
作者：
Gómez-Betancur I;Cortés N;Benjumea D;Osorio E;León F;Cutler SJ
Renealmia alpinia原产于美洲大陆，可以在墨西哥到巴西和加勒比群岛找到。它在哥伦比亚被称为“matandrea”，它通常用于传统医学，以治疗痛苦的疾病和疾病。基于其传统用途，它是感兴趣的，以评估这种植物及其次生代谢产物的药理作用。用甲醇和水提取野生和微繁殖R。通过高效薄层色谱法（HPTLC）对山姜（叶）进行化学比较。使用体内测定（Siegmund试验）检查这些提取物的抗伤害感受活性。此外，R.高良姜经分级分离，用色谱法分离得到纯化合物。通过核磁共振实验和光谱技术对分离的化合物进行了结构鉴定，并与文献数据进行了比较。评价纯化的化合物对阿片样物质和大麻素受体的体外结合亲和力。从该植物地上部分的二氯甲烷提取物中分离得到了醚菌酯（1）、柚皮素7，4 ′-二甲醚（2）、2′，6 ′-二羟基-4 ′-甲氧基查耳酮（3）、4-甲氧基-6-（2-苯乙烯基）-2H-吡喃-2-酮（4）、柚皮素7-甲醚（5）和3，5-庚烷，1，7-二苯基（6）。通过物理和光谱技术确定了它们的化学结构。野生和微繁殖植物提取物在小鼠中观察到的抗伤害性作用相似。从R.高良姜对阿片样物质或大麻素受体不显示亲和力。水提物和甲醇提取物。山姜在体内模型中提供抗伤害感受和镇痛作用。这些结果为解释为什么这种植物传统上用于疼痛管理提供了更多的见解。同时，这也是首次对该属植物化学成分进行全面研究的报道。山姜。
Renealmia alpinia is native to the American continent and can be found from Mexico to Brazil, and in the Caribbean islands. It is known as “matandrea” in Colombia, and it has been commonly used in traditional medicine to treat painful diseases and ailments. Based on its traditional uses, it is of interest to evaluate the pharmacologic effects of this plant and its secondary metabolites. Methanol and aqueous extracts of wild and micropropagated R. alpinia (leaves) were obtained and chemically compared by High Performance Thin Layer Chromatography (HPTLC). The antinociceptive activity of these extracts was examined using an in vivo assay (Siegmund test). Additionally, the dichloromethane extract of R. alpinia was fractionated and pure compounds were isolated by chromatographic methods. The structure elucidation of isolated compounds was performed by NMR experiments and spectroscopic techniques and comparison with the literature data. Purified compounds were evaluated for their in vitro binding affinity for opioids and cannabinoids receptors. The dichloromethane extract of the plant’s aerial part afforded sinostrobin (1), naringenin 7,4′-dimethyl ether (2), 2′,6′-dihydroxy-4′-methoxychalcone (3), 4-methoxy-6-(2-phenylethenyl)-2H-pyran-2-one (4), naringenin 7-methyl ether (5) and 3,5-heptanediol, 1,7-diphenyl (6), which were isolated using chromatographic methods. Their chemical structures were established by physical and spectroscopic techniques. The antinociceptive effects observed in mice by extracts of wild and micropropagated plants were similar. The compounds isolated from R. alpinia do not show affinity to opioid or cannabinoid receptors. Aqueous and methanol extracts of R. alpinia provide antinociceptive and analgesic effects in an in vivo model. These results contribute additional insight as to why this plant is traditionally used for pain management. Also, this is the first comprehensive report of a phytochemical study of R. alpinia.