Hydrogen peroxide-induced Ca2+ responses in CNS pericytes
Hydrogen peroxide-induced Ca2+ responses in CNS pericytes
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DOI：
10.1016/j.neulet.2007.01.039
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发表时间：
2007-04-06
影响因子：
2.5
通讯作者：
Iida, Mitsuo
中科院分区：
文献类型：
--
作者：
Kamouchi, Masahiro;Kitazono, Takanari;Iida, Mitsuo
目的：探讨中枢神经系统(CNS)周细胞内活性氧(ROS)与钙离子反应的相互作用。方法：用荧光钙指示剂Fura-2测定培养的CNS周细胞内钙离子浓度。结果：过氧化氢引起细胞内钙离子浓度依赖性升高，过氧化氢酶可完全抑制该作用。在应用过氧化氢的过程中，去除外部钙离子或加入尼卡地平(1mU M)并不影响钙离子的反应。细胞在无钙溶液中孵育并不能完全消除过氧化氢对细胞内钙离子的反应，但有轻微的抑制作用。Thapsigargin(1mM)耗竭细胞内钙离子并不改变细胞对过氧化氢的反应。酪氨酸激酶抑制剂金雀异黄素(100mM)或酪氨酸氨基转移酶A47(30mM)可显著降低过氧化氢引起的细胞内钙升高。结论：过氧化氢引起的细胞内钙升高主要是通过细胞内钙库释放，这可能受酪氨酸激酶的调节。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: The aims of the present study were to elucidate the interaction of reactive oxygen species (ROS) and Ca2+ response in central nervous system (CNS) pericytes. Methods: The intracellular Ca2+ concentration was measured using fluorescent Ca2+ indicator, fura-2, in cultured CNS pericytes. Results: Hydrogen peroxide evoked a dose-dependent increase in cytosolic Ca2+, which was completely inhibited by catalase. Removal of external Ca2+ or addition of nicardipine (1 mu M) during application of hydrogen peroxide did not affect Ca2+ response. Incubation of the cells in Ca2+ free solution did not abolish but slightly reduced Ca2+ response by hydrogen peroxide. Ca2+ response to hydrogen peroxide was not altered by the depletion of intracellular Ca2+ by thapsigargin (1 mu M). Pretreatment of the cells with tyrosine kinase inhibitor genistein (100 mu M) or tyrphostin A47 (30 mu M) significantly reduced Ca2+ increase by hydrogen peroxide. Conclusions: These results indicate that hydrogen peroxide evokes Ca2+ increase predominantly by release from intracellular Ca2+ store, which may be regulated by tyrosine kinases. (c) 2007 Elsevier Ireland Ltd. All rights reserved.