Mapping the active site of meprin-A with peptide substrates and inhibitors.
Mapping the active site of meprin-A with peptide substrates and inhibitors.
复制标题
使用肽底物和抑制剂绘制 meprin-A 的活性位点。
DOI：
10.1021/bi00098a029
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Bond,JS
中科院分区：
文献类型：
--
作者：
Wolz,RL;Harris,RB;Bond,JS
材料与方法酶。从冷冻的ICR雄性小鼠肾（罗克兰农场公司，Gilbertsville，PA），如前所述（Wolz & Bond，1990）。通过Lowry et al.（1951），用牛血清白蛋白作为标准，或基于A0 1%= 1.0通过280 nm处的吸光度估计。表I和II中列出的肽和表V中的氨基酸异羟肟酸盐购自Sigma Chemical Co. (St. Louis，MO）。在表III和IV中列出的显色和荧光底物通过固相法在一个反应器上合成。
Materials and MethodsEnzyme. Meprin-A was purified from frozen ICR male mouse kidneys (Rockland Farms, Inc., Gilbertsville, PA) as previously described (Wolz & Bond, 1990). Protein concentration was determined by the method of Lowry et al.(1951), with bovine serum albumin as a standard, or estimated by absorbance at 280 nm on the basis of A0 l%= 1.0.Peptides. The peptides listed in Tables I and II and the amino acid hydroxamates in Table V were purchased from the Sigma Chemical Co.(St. Louis, MO). The chromogenic and fluorogenic substrates listed in Tables III and IV were synthesized by solid-phase methods on a