Human immunodeficiency virus type 1 (HIV-1) antigen secretion by latently infected resting CD4+ T lymphocytes from HIV-1-infected individuals
Human immunodeficiency virus type 1 (HIV-1) antigen secretion by latently infected resting CD4+ T lymphocytes from HIV-1-infected individuals
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DOI：
10.1128/jvi.78.19.10536-10542.2004
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发表时间：
2004-10-01
影响因子：
5.4
通讯作者：
Vendrell, JP
中科院分区：
文献类型：
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作者：
Fondere, JM;Petitjean, G;Vendrell, JP
在携带人类免疫缺陷病毒1型（HIV-1）的静息CD 4（+）T淋巴细胞中，有复制能力的病毒在对高效抗逆转录病毒治疗（HAART）有反应的患者中持续存在。这个小的潜在储库代表每个患者10（3）至10（7）个细胞。然而，HIV-1 DNA阳性静息CD 4（+）T细胞在体外CD 4（+）-T细胞多克隆刺激后转化为HIV-1抗原分泌细胞（HIV-1-Ag-SC）的效率尚未得到令人满意的评价。通过使用HIV-1抗原酶联免疫斑点试验，在25名血浆病毒载量为1000 mg/kg的患者中，每10（6）个CD 4（+）静息T细胞中定量检测到8个HIV-1-Ag-SC。
In resting CD4(+) T lymphocytes harboring human immunodeficiency virus type 1 (HIV-1), replication-competent virus persists in patients responding to highly active antiretroviral therapy (HAART). This small latent reservoir represents between 10(3) and 10(7) cells per patient. However, the efficiency of HIV-1 DNA-positive resting CD4(+) T cells in converting to HIV-1-antigen-secreting cells (HIV-1-Ag-SCs) after in vitro CD4(+)-T-cell polyclonal stimulation has not been satisfactorily evaluated. By using an HIV-1-antigen enzyme-linked immunospot assay, 8 HIV-1-Ag-SCs per 10(6) CD4(+) resting T cells were quantified in 25 patients with a plasma viral load of