Expression of organic cation transporter OCTN1 in hematopoietic cells during erythroid differentiation
Expression of organic cation transporter OCTN1 in hematopoietic cells during erythroid differentiation
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DOI：
10.1016/j.exphem.2004.08.009
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发表时间：
2004-12-01
影响因子：
2.6
通讯作者：
Tamai, I
中科院分区：
文献类型：
--
作者：
Kobayashi, D;Aizawa, S;Tamai, I
目标。有机阳离子/肉碱转运蛋白OCTN1(SLC22A4)具有相对广泛的组织分布，以pH依赖的方式转运有机阳离子。然而，它的生理作用仍有待阐明。为了解OCTN1的生理作用，对OCTN1在人和小鼠体内的组织表达进行了研究。用逆转录聚合酶链式反应、Western印迹和流式细胞术检测OCTN1在不同组织和血细胞中的表达。RT-PCR检测到小鼠肾脏、平滑肌和脾、骨髓等造血组织中有OCTN1基因的表达。进一步的研究集中在OCTN1在不同类型血细胞中的表达。在小鼠骨髓髓系细胞中检测到OCTN1基因，而在淋巴系细胞中未检测到。红细胞标志物TER119阳性的骨髓有核细胞表达OCTN1。同样，OCTN1在从人脐血中获得的血糖素A阳性的红系细胞中也有强烈的表达。在Western印迹分析中，在分离的成熟小鼠外周血红细胞中检测到OCTN1蛋白。进一步的RT-PCR和流式细胞仪分析表明，OCTN1在培养的血糖素A阳性和阴性的红系细胞中均有表达。这些发现提示OCTN1转运红系分化、成熟和/或生长所需的化合物(S)。本研究表明，OCTN1与髓系细胞相关，而不是与淋巴系细胞相关，尤其是与红系细胞从未成熟红系细胞向外周成熟红系细胞过渡阶段有关。(C)2004年国际实验血液学学会。由爱思唯尔公司出版。
Objective. Organic cation/carnitine transporter, OCTN1 (SLC22A4) shows a relatively broad tissue distribution and transports organic cations in a pH-dependent manner. However, its physiological role remains to be clarified. To understand the physiological role of OCTN1, tissue expression of OCTN1 in human and mice was characterized.Methods. Expression of OCTN1 in various tissues and blood cells was examined by reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and flow cytometry analysis.Results. Mouse OCTN1 mRNA was detected in kidney, smooth muscle, and hematopoietic tissues, such as spleen and bone marrow, by RT-PCR analysis. Further study focused on expression of OCTN1 in various types of blood cells. OCTN1 mRNA was detected in myeloid cells in mouse bone marrow, but not in lymphoid cells. Bone marrow nuclear cells positive for TER119, an erythrocyte marker, showed strong expression of OCTN1. Similarly, OCTN1 was strongly expressed in glycophorin A-positive erythroid cells obtained from human cord blood. In Western blot analysis, OCTN1 protein was detected in isolated mouse mature peripheral erythrocytes. Further analysis by RT-PCR and flow cytometry showed OCTN1 was expressed in both glycophorin A-positive and negative erythroid cells after cultivation. These findings suggested that OCTN1 transports compound(s) that are required for erythroid differentiation, maturation, and/or growth.Conclusion. The present study demonstrated that OCTN1 is associated with myeloid cells rather than lymphoid cells, and especially with erythroid-lineage cells at the transition stage from immature erythroid cells to peripheral mature erythrocytes. (C) 2004 International Society for Experimental Hematology. Published by Elsevier Inc.