Differences in SpeB protease activity among group A streptococci associated with superficial, invasive, and autoimmune disease.
Differences in SpeB protease activity among group A streptococci associated with superficial, invasive, and autoimmune disease.
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DOI：
10.1371/journal.pone.0177784
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Bessen DE
中科院分区：
文献类型：
--
作者：
Ly AT;Noto JP;Walwyn OL;Tanz RR;Shulman ST;Kabat W;Bessen DE
分泌的半胱氨酸蛋白酶SpeB是A组链球菌（GAS）的重要毒力因子，其中SpeB活性在菌株之间变化很大。为了确定SpeB活性与疾病相关的程度，从患有咽炎、脓疱病、侵袭性疾病或急性风湿热（ARF）的患者中回收GAS微生物，并使用严格的采样标准选择用于分析;通过酪蛋白消化测定法测试>300个GAS分离株的SpeB活性，并将每个GAS分离株评分为SpeB生产者或非生产者。从ARF患者中回收的GAS（41.5% SpeB-非生产者）与咽炎（20.5%）、浸润性疾病（16.7%）和脓疱（5.5%）相比，SpeB生产具有高度显著的统计学差异（p < 0.01）。SpeB活性的差异咽炎和脓疱病毒分离株也是显着的，而咽炎与侵袭性分离株显示没有显着差异。在ARF相关的分离株中，SpeB非生产者的数量不成比例地增加，这可能表明许多致风湿性菌株的转录程序发生了改变，和/或SpeB在气体触发的自身免疫中起保护作用。
The secreted cysteine proteinase SpeB is an important virulence factor of group A streptococci (GAS), whereby SpeB activity varies widely among strains. To establish the degree to which SpeB activity correlates with disease, GAS organisms were recovered from patients with pharyngitis, impetigo, invasive disease or acute rheumatic fever (ARF), and selected for analysis using rigorous sampling criteria; >300 GAS isolates were tested for SpeB activity by casein digestion assays, and each GAS isolate was scored as a SpeB-producer or non-producer. Highly significant statistical differences (p < 0.01) in SpeB production are observed between GAS recovered from patients with ARF (41.5% SpeB-non-producers) compared to pharyngitis (20.5%), invasive disease (16.7%), and impetigo (5.5%). SpeB activity differences between pharyngitis and impetigo isolates are also significant, whereas pharyngitis versus invasive isolates show no significant difference. The disproportionately greater number of SpeB-non-producers among ARF-associated isolates may indicate an altered transcriptional program for many rheumatogenic strains and/or a protective role for SpeB in GAS-triggered autoimmunity.