Laboratory diagnosis of four recent sporadic cases of community-acquired SARS, Guangdong Province, China.
Laboratory diagnosis of four recent sporadic cases of community-acquired SARS, Guangdong Province, China.
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DOI：
10.3201/eid1010.040445
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发表时间：
2004-10
影响因子：
11.8
通讯作者：
SARS Diagnosis Working Group
中科院分区：
文献类型：
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作者：
Liang G;Chen Q;Xu J;Liu Y;Lim W;Peiris JS;Anderson LJ;Ruan L;Li H;Kan B;Di B;Cheng P;Chan KH;Erdman DD;Gu S;Yan X;Liang W;Zhou D;Haynes L;Duan S;Zhang X;Zheng H;Gao Y;Tong S;Li D;Fang L;Qin P;Xu W;SARS Diagnosis Working Group
通过四个实验室的合作，确定了四例SARS相关冠状病毒感染的散发病例。对2003年12月16日至2004年1月8日广州市发生的4例严重急性呼吸综合征（SARS）病例进行了调查。从这些患者身上采集的临床标本由中国的省级和国家实验室以及世界卫生组织SARS参考和验证实验室网络的成员进行检测，共同努力识别和确认SARS相关冠状病毒（SARS-CoV）感染。虽然没有从任何患者中分离出SARS-CoV，但通过逆转录-聚合酶链反应检测，3名患者的标本中病毒RNA呈阳性，所有患者的SARS-CoV特异性抗体都有可检测的升高。这项研究显示了合作的多实验室响应诊断SARS的有效性。
Four sporadic cases of SARS-associated coronavirus infection were identified through collaboration of four laboratories. Four cases of severe acute respiratory syndrome (SARS) that occurred from December 16, 2003, to January 8, 2004, in the city of Guangzhou, Guangdong Province, China, were investigated. Clinical specimens collected from these patients were tested by provincial and national laboratories in China as well as members of the World Health Organization SARS Reference and Verification Laboratory Network in a collaborative effort to identify and confirm SARS-associated coronavirus (SARS-CoV) infection. Although SARS-CoV was not isolated from any patient, specimens from three patients were positive for viral RNA by reverse transcription–polymerase chain reaction assay, and all patients had detectable rises in SARS-CoV–specific antibodies. This study shows the effectiveness of a collaborative, multilaboratory response to diagnose SARS.