Expression of type X collagen mRNA levels in embryonic chick sternum during development.
Expression of type X collagen mRNA levels in embryonic chick sternum during development.
复制标题
发育过程中胚胎鸡胸骨中 X 型胶原蛋白 mRNA 水平的表达。
DOI：
10.1016/0006-291x(86)90342-6
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Jimenez,SA
中科院分区：
文献类型：
--
作者：
Reginato,AM;Lash,JW;Jimenez,SA
胚胎鸡胸骨软骨在发育过程中 X 型胶原生物合成表现出深刻的空间和时间变化。这种胶原蛋白的产生仅限于假定的钙化区域，直到第 43 阶段才获得其表达。为了检查 X 型胶原蛋白生物合成表达发育变化的调节机制，我们采用无细胞翻译系统测定了可翻译的 X 型原胶原 mRNA 的水平。我们发现能够指导 X 型胶原蛋白合成的 mRNA 只存在于注定要经历钙化的软骨中，并且其水平在所有发育阶段几乎相同。这些发现表明，胚胎鸡胸骨中 X 型胶原蛋白的表达是在翻译水平上决定的。
Embryonic chick sternum cartilage exhibits profound spatial and temporal changes in Type X collagen biosynthesis during development. Production of this collagen is confined to the presumptive calcification region and its expression is not acquired until stage 43. To examine the mechanisms responsible for regulation of developmental changes in biosynthetic expression of Type X collagen, we determined the levels of translatable Type X procollagen mRNA employing a cell-free translation system. We found that mRNA capable of directing Type X collagen synthesis was present exclusively in cartilage destined to undergo calcification and that its levels were nearly equivalent at all stages of development. These findings suggest that expression of Type X collagen in embryonic chick sternum is determined at the translational level.