Construction of amperometric immunosensors based on the electrogeneration of a permeable biotinylated polypyrrole film
Construction of amperometric immunosensors based on the electrogeneration of a permeable biotinylated polypyrrole film
复制标题
DOI：
10.1021/ac049413z
复制
发表时间：
2004-11-15
影响因子：
7.4
通讯作者：
Marks, RS
中科院分区：
文献类型：
--
作者：
Ionescu, RE;Gondran, C;Marks, RS
当霍乱毒素 B 亚基生物识别实体与与吡咯乳糖酰胺单体共聚的电生生物素化聚吡咯膜连接时，构建针对霍乱抗毒素免疫球蛋白的电流免疫传感器具有提高的灵敏度。对于电活性物质的渗透，该共聚物表现出比生物素化聚吡咯或多酚膜更大的膜渗透性。因此，当使用氢醌测定免疫测定的 HRP 标记物的存在时，电活性醌的产生显示出更快地渗透到电极，从而提供更快的响应时间。
The construction of amperometric immunosensors to cholera antitoxin immunoglobulins were shown to have improved sensitivity when the cholera toxin B subunit biorecognition entity was linked to an electrogenerated biotinylated polypyrrole film copolymerized with pyrrolelactobionamide monomer. The copolymer exhibits greater film permeability than biotinylated polypyrrolic or polyphenolic films for the permeation of electroactive species. Hence, when the presence of the HRP marker of the immunoassay was determined using hydroquinone, the production of electroactive quinone was shown to permeate faster to the electrode, thus providing a faster response time.