"Reactive" probe for hydrogen sulfite: "turn-on" fluorescent sensing and bioimaging application
"Reactive" probe for hydrogen sulfite: "turn-on" fluorescent sensing and bioimaging application
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DOI：
10.1039/c3tb20159g
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Li, Zhen
中科院分区：
文献类型：
--
作者：
Cheng, Xiaohong;Jia, Huizhen;Li, Zhen
利用醛的亲核加成反应，以分子内电荷转移（ICT）为信号机制，合成了一种亚硫酸氢盐的“开启”荧光探针（C1）。加入HSO 3-离子后，探针显示出明显的荧光变化，从无发射到强绿色荧光。C_1对亚硫酸氢有高灵敏度的响应，检测限低至3.0 μ M。C1对亚硫酸氢根的选择性比其它常见阴离子高，因此C1被成功地应用于开启荧光法检测HeLa细胞中的亚硫酸氢根。
Taking advantage of the special nucleophilic addition reaction with aldehyde, a "switching-on" fluorescent probe (C1) for hydrogen sulfite was synthesized using intramolecular charge transfer (ICT) as a signal mechanism. Upon the addition of HSO3- ions, the probe displayed apparent fluorescence changes from non-emission to strong green fluorescence. C1 gave response to hydrogen sulfite with high sensitivity and the detection limit was determined to be as low as 3.0 mu M. In addition to its high selectivity for hydrogen sulfite rather than other common anions, C1 was successfully applied to the detection of hydrogen sulfite in HeLa cells with turn-on fluorescent methods.