Cell wall layers delimit cell groups derived from cell division in the foliose trebouxiophycean alga Prasiola japonica
Cell wall layers delimit cell groups derived from cell division in the foliose trebouxiophycean alga Prasiola japonica
复制标题
细胞壁层界定了叶状三环藻类海藻Prasiola japonica中细胞分裂衍生的细胞群
DOI：
10.1007/s00709-018-1206-4
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发表时间：
2018
期刊：
影响因子：
2.9
通讯作者：
Sekida Satoko
中科院分区：
文献类型：
--
作者：
Mine Ichiro;Kinoshita Urara;Kawashima Shigetaka;Sekida Satoko
日本海藻(Prasiola Japan Onica)叶状叶状体中的细胞明显发育成2个 × 2细胞群，由两个双细胞群组成，每个细胞群是最近一次细胞分裂的一对衍生细胞。本文研究了赤潮藻细胞壁的结构特征。用组织化学方法观察细胞群的形成。硫代荧光白染色的薄层细胞层分别包裹着2-细胞和4-细胞组，从而将它们与相邻的细胞组分开，相邻的4-细胞组之间的硫系荧光素阴性间隙被大豆凝集素、茉莉素和与荧光素结合的Vicia villosalectin等凝集素特异性地染色。这些结果表明，在连续两次细胞分裂中，亲本细胞中存在的硫代荧光白阳性细胞壁层在结构上区别于该藻中的其他两细胞群和四细胞群。此外，这些结果还表明，银白色负性间隙的细胞壁成分可能通过细胞群的侧向联合而促进了平面叶状体的形成。
The cells in the foliose thallus of trebouxiophycean algaPrasiola japonicaapparently develop into 2 × 2 cell groups composed of two two-celled groups, each of which is a pair of derivative cells of the latest cell division. In the present study, the structural features of cell walls of the algaP. japonicaconcerning the formation of the cell groups were investigated using histochemical methods. Thin cell layers stained by Calcofluor White appeared to envelope the two-celled and four-celled groups separately and, hence, separated them from neighboring cell groups, and the Calcofluor White-negative gaps between neighboring four-celled groups were specifically stained by lectins, such as soybean agglutinin, jacalin, andVicia villosalectin conjugated with fluorescein. These results indicated that the Calcofluor White-positive cell wall layer of parent cell that existed during two successive cell divisions structurally distinguished two-celled and four-celled groups from others in this alga. Moreover, the results suggested that the cell wall components of the Calcofluor White-negative gaps would possibly contribute to the formation of the planar thallus through lateral union of the cell groups.