Rodent Neural Progenitor Cells Support Functional Recovery after Cervical Spinal Cord Contusion
Rodent Neural Progenitor Cells Support Functional Recovery after Cervical Spinal Cord Contusion
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DOI：
10.1089/neu.2017.5244
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发表时间：
2018-03-01
影响因子：
4.2
通讯作者：
Tuszynski, Mark Henry
中科院分区：
文献类型：
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作者：
Brock, John Hoffman;Graham, Lori;Tuszynski, Mark Henry
以前，我们和其他人已经表明，啮齿动物神经祖细胞（NPC）可以支持颈椎和胸椎横断损伤后的功能恢复。为了将这些观察结果扩展到更临床相关的脊髓损伤模型，我们在Fischer 344大鼠中进行了单侧颈中段挫伤。两周后，将E14衍生的同基因脊髓衍生的多能NPC植入病变腔中。对照组动物接受无移植物或成纤维细胞移植物。NPC分化成所有三种神经谱系（神经元、星形胶质细胞、少突胶质细胞），并在病变的尾侧和喙侧将轴突稳健地延伸到宿主脊髓中。移植物衍生的轴突生长到宿主灰质中，并与宿主神经元并列表达突触蛋白。与两个对照组相比，接受NPC移植的动物表现出前肢运动功能的显著恢复（方差分析p
Previously, we and others have shown that rodent neural progenitor cells (NPCs) can support functional recovery after cervical and thoracic transection injuries. To extend these observations to a more clinically relevant model of spinal cord injury, we performed unilateral midcervical contusion injuries in Fischer 344 rats. Two-weeks later, E14-derived syngeneic spinal cord-derived multi-potent NPCs were implanted into the lesion cavity. Control animals received either no grafts or fibroblast grafts. The NPCs differentiated into all three neural lineages (neurons, astrocytes, oligodendrocytes) and robustly extended axons into the host spinal cord caudal and rostral to the lesion. Graft-derived axons grew into host gray matter and expressed synaptic proteins in juxtaposition with host neurons. Animals that received NPC grafts exhibited significant recovery of forelimb motor function compared with the two control groups (analysis of variance p