Biodegradation of marine oil spill residues using aboriginal bacterial consortium based on Penglai 19-3 oil spill accident, China.
Biodegradation of marine oil spill residues using aboriginal bacterial consortium based on Penglai 19-3 oil spill accident, China.
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DOI：
10.1016/j.ecoenv.2018.04.059
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发表时间：
2018-09
影响因子：
6.8
通讯作者：
Chuanyuan Wang;Xing Liu;Jie Guo;Yingchun Lv;Yuanwei Li
中科院分区：
文献类型：
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作者：
Chuanyuan Wang;Xing Liu;Jie Guo;Yingchun Lv;Yuanwei Li
生物修复技术是处理溢油污染的一种有效方法，主要是利用土著细菌进行修复。研究了渤海蓬莱19-3石油平台沉积物中微生物共培养对原油的生物降解作用。还讨论了烷基萘、烷基菲和烷基二苯并噻吩异构体对生物降解的相对敏感性。结果表明，未处理的微宇宙（S-BR 1）和表面活性剂处理的微宇宙（S-BR 2）对沉积物中总石油烃（TPH）的相对降解率分别为43.56%和51.29%。TPH降解结果表明，饱和烃含量明显降低（降解率为67.85-77.29%），芳烃含量略有降低（降解率为47.13-57.21%），胶质和沥青质含量变化不大。S-BR 1和S-BR 2样品对烷基萘、烷基菲和烷基二苯并噻吩的生物降解率分别为1.28-84.43%和42.56- 86.67%。添加Tween 20的微宇宙培养物在沉积物中的原油降解效率高于未处理的微宇宙培养物。生物降解和选择性消耗不仅受热力学控制，而且与异构体的立体结构有关。在这项研究中揭示的细菌群落的溢油残留物的生物降解的信息将是有用的，在制定战略，分散在海洋生态系统中的原油生物修复。
Bioremediation, mainly by indigenous bacteria, has been regarded as an effective way to deal with the petroleum pollution after an oil spill accident. The biodegradation of crude oil by microorganisms co-incubated from sediments collected from the Penglai 19-3 oil platform, Bohai Sea, China, was examined. The relative susceptibility of the isomers of alkylnaphthalenes, alkylphenanthrenes and alkyldibenzothiophene to biodegradation was also discussed. The results showed that the relative degradation values of total petroleum hydrocarbon (TPH) are 43.56% and 51.29% for sediments with untreated microcosms (S-BR1) and surfactant-treated microcosms (S-BR2), respectively. TPH biodegradation results showed an obvious decrease in saturates (biodegradation rate: 67.85–77.29%) and a slight decrease in aromatics (biodegradation rate: 47.13–57.21%), while no significant difference of resins and asphaltenes was detected. The biodegradation efficiency of alkylnaphthalenes, alkylphenanthrenes and alkyldibenzothiophene for S-BR1 and S-BR2 samples reaches 1.28–84.43% and 42.56–86.67%, respectively. The efficiency of crude oil degradation in sediment with surfactant-treated microcosms cultures added Tween 20, was higher than that in sediment with untreated microcosms. The biodegradation and selective depletion is not only controlled by thermodynamics but also related to the stereochemical structure of individual isomer compounds. Information on the biodegradation of oil spill residues by the bacterial community revealed in this study will be useful in developing strategies for bioremediation of crude oil dispersed in the marine ecosystem.