Fractional laser-assisted drug delivery: Laser channel depth influences biodistribution and skin deposition of methotrexate
Fractional laser-assisted drug delivery: Laser channel depth influences biodistribution and skin deposition of methotrexate
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DOI：
10.1002/lsm.22484
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发表时间：
2016-07-01
影响因子：
2.4
通讯作者：
Haedersdal, M.
中科院分区：
文献类型：
--
作者：
Taudorf, E. H.;Lerche, C. M.;Haedersdal, M.
背景和目的：消融性分数激光（AFXL）有助于局部甲氨蝶呤（MTX）的输送。本研究调查局部MTX delivery.Materials和MethodsMTX（1% [w/v]）的激光通道深度的影响扩散21小时，通过AFXL暴露的猪皮肤在体外Franz细胞（n=120）。2，940 nm AFXL在表皮（11 mJ/通道，MAZ-E）、浅层真皮（26 mJ/通道，MAZ-DS）和中层真皮（256 mJ/通道，MAZ-DM）中产生微观消融区（MAZ）。高效液相色谱法（HPLC）被用来量化MTX沉积在全层皮肤，生物分布在特定的皮肤水平，和透皮渗透。用荧光显微镜观察紫外线激活的MTX荧光（254 nm）和半定量MTX在皮肤中的分布。
Background and ObjectiveAblative fractional laser (AFXL) facilitates delivery of topical methotrexate (MTX). This study investigates impact of laser-channel depth on topical MTX-delivery.Materials and MethodsMTX (1% [w/v]) diffused for 21 hours through AFXL-exposed porcine skin in in vitro Franz Cells (n=120). A 2,940nm AFXL generated microscopic ablation zones (MAZs) into epidermis (11mJ/channel, MAZ-E), superficial-dermis (26mJ/channel, MAZ-DS), and mid-dermis (256mJ/channel, MAZ-DM). High performance liquid chromatography (HPLC) was used to quantify MTX deposition in full-thickness skin, biodistribution profiles at specific skin levels, and transdermal permeation. Fluorescence microscopy was used to visualize UVC-activated MTX-fluorescence (254nm) and semi-quantify MTX distribution in skin.ResultsAFXL increased topical MTX-delivery (P