PHYSIOLOGY AND ULTRASTRUCTURE OF ELECTROTONIC JUNCTIONS .4. MEDULLARY ELECTROMOTOR NUCLEI IN GYMNOTID FISH
PHYSIOLOGY AND ULTRASTRUCTURE OF ELECTROTONIC JUNCTIONS .4. MEDULLARY ELECTROMOTOR NUCLEI IN GYMNOTID FISH
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DOI：
10.1152/jn.1967.30.2.236
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发表时间：
1967-01-01
影响因子：
2.5
通讯作者：
NAKAJIMA, Y
中科院分区：
文献类型：
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作者：
BENNETT, MVL;PAPPAS, GD;NAKAJIMA, Y
方法光学和电子显微镜技术以及电生理学操作如前所述（15，19，21）。大多数动物都是被圈养的。这种处理阻断了电蚀斑的放电，尽管电蚀斑系统的活性继续。参考反应，从身体后部的电极获得高增益记录，由下行束和脊髓神经元的活动引起。为了简单起见，这种反应将被认为等同于电器官的放电。在所有实验中，示波器由器官放电或细胞内记录的电位触发。通过使用延迟或扩展的扫描刺激可以在器官周期中的任何固定时间和器官频率的某个约数（通常为1或2/组）呈现。细胞内刺激总是使用桥接电路中的单个电极进行（21）。如前所述，这种方法需要间接测量输入电阻。此外，电极电阻和电位的变化可能是由极化电流产生的，结果是在脉冲期间膜电位的变化被掩盖。
METHODSTechniques for light and electron microscopy and for electrophysiological procedures were as previously described(15, 19, 21). Most animals were curarized. This treatment blocked discharge of the electroplaques, although activity of the electromotor system continued. A reference response, obtained by high-gain recording from electrodes in the posterior of the body, resulted from activity of the descending tracts and spinal neurons. For simplicity in presentation this response will be considered equivalent to discharge of the electric organ. In all experiments the oscilloscope was triggered by the organ discharge or by an intracellularly recorded potential. By use of a delayed or expanded sweep stimuli could be presented at any fixed time in the organ cycle and at some submultiple of the organ frequency, usually I or 2/set. Intracellular stimulation was always carried aut using a single electrode in a bridge circuit(21). As discussed previously this method requires that input resistance be measured indirectly. Also, changes in electrode resistance and potential may be produced by polarizing current with the result that membrane potential changes during the pulses are obscured.