Clearance of I-125-labeled interleukin-6 from brain into blood following intracerebroventricular injection in rats
Clearance of I-125-labeled interleukin-6 from brain into blood following intracerebroventricular injection in rats
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DOI：
10.1210/en.138.11.4830
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发表时间：
1997-11-01
期刊：
影响因子：
4.8
通讯作者：
Reichlin, S
中科院分区：
文献类型：
--
作者：
Chen, GJ;Castro, WL;Reichlin, S
为验证脑内诱导的白细胞介素6（IL-6）可释放到外周血的假说，将~（125）I标记的IL-6注入大鼠侧脑室，动态监测其在外周血中的浓度。酸可沉淀示踪剂在注射后5分钟内出现，并按照一级动力学（分数速率，0.0116 +/- 0.0022/min）进入血液。侧脑室注射与静脉注射的曲线下面积比较表明，注入侧脑室的示踪剂在4小时内出现在血液中的占37.1-46.5%。冰冷IL-6至少部分通过静脉引流排出（在两个独立组中，上级矢状窦/主动脉浓度梯度为1.47 +/- 0.23和3.05 +/- 0.87）。预先冰冷注射人IL-1 β（100 ng）不会改变放射性碘标记的IL-6从脑中的降解或排出速率。这些研究表明，在大脑中产生的相对高比例的IL-6进入外周循环。从布兰到血液的IL-6的直接分泌可能是大脑调节外周代谢、内分泌和免疫活性的机制。
To test the hypothesis that interleukin-6 (IL-6) induced within the brain can be released into peripheral blood, I-125-labeled IL-6 was injected into the lateral cerebral ventricle of rats, and its concentration in peripheral blood followed serially. Acid-precipitable tracer appeared within 5 min of injection and entered the blood following first-order kinetics (fractional rate, 0.0116 +/- 0.0022/min). Comparison of areas under the curve of intracerebroventricular (icv) vs. iv injection showed that 37.1-46.5% of tracer injected into the lateral cerebral ventricle appeared in the blood over a 4-h period. icy IL-6 exits at least in part via venous drainage (superior sagittal sinus/aortic concentration gradient was 1.47 +/- 0.23 and 3.05 +/- 0.87 ill two separate groups). Prior icy injection of human IL-1 beta (100 ng) did not alter rate of degradation or of exit of radioiodine-labeled IL-6 from the brain. These studies indicate that a relatively high proportion of IL-6 that arises in the brain enters the peripheral circulation. Direct secretion of IL-6 from blain to blood may be a mechanism by which the brain modifies peripheral metabolic, endocrine, and immune activity.